| Literature DB >> 4607021 |
T Kornberg, A Lockwood, A Worcel.
Abstract
Semi-conservative DNA synthesis is observed when the isolated, folded E. coli chromosome is supplemented with a DNA-free, soluble enzyme fraction, the four deoxynucleoside 5'-triphosphates, ATP, and Mg(++). The DNA synthesized in vitro remains associated with the folded chromosome during sedimentation through neutral sucrose, but is released as small DNA fragments in alkali. Sealing of these replicative intermediates to the chromosome requires the presence of both E. coli DNA polymerase I (EC 2.7.7.7) and DNA ligase (EC 6.5.1.2).Entities:
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Year: 1974 PMID： 4607021 PMCID： PMC388648 DOI： 10.1073/pnas.71.8.3189
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205