| Literature DB >> 770443 |
Abstract
Nuclear bodies were isolated from Escherichia coli spheroplasts by two different lysis procedures. Their lipid and protein content and the superhelix density was measured. The preparations differed mainly in respect to the amount of the attached membrane material, which seems to be an essential factor in maintaining the stability of the nuclear bodies. The superhelix density was found to be higher than that of naturally occurring, covalently closed, circular deoxyribonucleic acids. The influence of the preparative method on the superhelicity was comparatively small.Entities:
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Year: 1976 PMID: 770443 PMCID: PMC233260 DOI: 10.1128/jb.126.1.64-71.1976
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490