Literature DB >> 460180

Postribosomal complexes containing eukaryotic initiation factor eIF-2.

H Amesz, T Haubrich, H O Voorma.   

Abstract

Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with other protein(s) with a sedimentation value of 16-20S. This complexed fraction contains besides eIF-2 another activity which exhibits a profound stimulation on amino acid incorporation in crude lysates and appears to counteract the heme-regulated inhibitor.

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Year:  1979        PMID： 460180     DOI： 10.1007/bf00777498

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  12 in total

1.  Purification and characterization of initiation factor IF-E2 from rabbit reticulocytes.

Authors:  R Benne; C Wong; M Luedi; J W Hershey
Journal:  J Biol Chem       Date:  1976-12-10       Impact factor: 5.157

2.  Purification and characterization of initiation factor IF-E3 from rabbit reticulocytes.

Authors:  R Benne; J W Hershey
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

3.  Purification and characterization of two initiation factors required for maximal activity of a highly fractionated globin mRNA translation system.

Authors:  B Safer; S L Adams; W M Kemper; K W Berry; M Lloyd; W C Merrick
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

4.  Interaction of poly(A) and mRNA with eukaryotic initiator met-tRNA-f binding factor: identification of this activity on reticulocyte ribonucleic acid protein particles.

Authors:  J G Hellerman; D A Shafritz
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

5.  Nonribosomal proteins associated with eukaryotic native small ribosomal subunits.

Authors:  C Freienstein; G Blobel
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

6.  Purification and physical properties of homogeneous initiation factor MP from rabbit reticulocytes.

Authors:  B Safer; W F Anderson; W C Merrick
Journal:  J Biol Chem       Date:  1975-12-10       Impact factor: 5.157

7.  Initiation of protein synthesis in eukaryotes. Binding to Sepharose-heparin and partial purification of initiation factors from Krebs II ascites cells.

Authors:  C Van der Mast; A Thomas; H Goumans; H Amesz; H O Voorma
Journal:  Eur J Biochem       Date:  1977-05-16

8.  Correlation of specific coding sequences with specific proteins associated in untranslated cytoplasmic messenger ribonucleoprotein complexes of duck erythroblasts.

Authors:  A Vincent; O Civelli; J F Buri; K Scherrer
Journal:  FEBS Lett       Date:  1977-05-15       Impact factor: 4.124

9.  A protein of molecular weight 78,000 bound to the polyadenylate region of eukaryotic messenger RNAs.

Authors:  G Blobel
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

10.  Comparison of mRNA binding by Met-tRNAf binding protein and mRNA-associated proteins.

Authors:  A Barrieux; M G Rosenfeld
Journal:  J Biol Chem       Date:  1977-01-10       Impact factor: 5.157
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