| Literature DB >> 4599954 |
Abstract
Recent refinements simplify methods for P22 transduction in Salmonella and allow improved recovery of phage-free transductional clones. The methods include use of: integration- and lysis-defective phage mutants, heat-killed bacteria to eliminate free phage, direct plating of phage and bacteria, replica-plating for detection of phage content of individual clones, improved broth for phage growth, and procurement of high titer phage from P22 lysogens.Entities:
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Year: 1974 PMID: 4599954 PMCID: PMC1213092
Source DB: PubMed Journal: Genetics ISSN: 0016-6731 Impact factor: 4.562