Literature DB >> 4598303

Composition of mammalian ribosomal subunits: a re-evaluation.

E H McConkey.   

Abstract

A method for preparation of highly active mammalian ribosomal subunits is described, which yields 60S subunits containing no more than 33% protein. It is suggested that the composition of these subunits corresponds closely to that of Escherichia coli 50S subunits. Data on the composition of bacterial and mammalian ribosomal subunits recovered from CsCl are given. It is shown that the commonly employed assumptions about the relationship between the buoyant density of a particle in CsCl and its protein content are in error. The composition of ribosomal subunits cannot ordinarily be calculated from the buoyant density in CsCl.

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Year:  1974        PMID： 4598303      PMCID： PMC388232          DOI： 10.1073/pnas.71.4.1379

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Isopycnic separation of subcellular components from poliovirus-infected and normal HeLa cells.

Authors:  D Baltimore; A S Huang
Journal:  Science       Date:  1968-11-01       Impact factor: 47.728

2.  The dissociation of rat liver ribosomes by ethylenediaminetetraacetic acid; molecular weights, chemical composition, and buoyant densities of the subunits.

Authors:  M G Hamilton; M E Ruth
Journal:  Biochemistry       Date:  1969-03       Impact factor: 3.162

3.  The ribosomal proteins of Escherichia coli. II. Chemical and physical characterization of the 30S ribosomal proteins.

Authors:  G R Craven; P Voynow; S J Hardy; C G Kurland
Journal:  Biochemistry       Date:  1969-07       Impact factor: 3.162

4.  Synthesis of phage-specific alpha- and beta-glucosyl transferases directed by T-even DNA in vitro.

Authors:  L M Gold; M Schweiger
Journal:  Proc Natl Acad Sci U S A       Date:  1969-03       Impact factor: 11.205

5.  Molecular weights of ribosomal RNA in relation to evolution.

Authors:  U E Loening
Journal:  J Mol Biol       Date:  1968-12       Impact factor: 5.469

6.  The requirements for specific sRNA binding by ribosomes.

Authors:  C G Kurland
Journal:  J Mol Biol       Date:  1966-06       Impact factor: 5.469

7.  Studies on the structure of ribosomes. II. Stepwise dissociation of protein from ribosomes by caesium chloride and the re-assembly of ribosome-like particles.

Authors:  M I Lerman; A S Spirin; L P Gavrilova; V F Golov
Journal:  J Mol Biol       Date:  1966-01       Impact factor: 5.469

8.  Isolation and physical properties of the ribosomal ribonucleic acid of Escherichia coli.

Authors:  W M Stanley; R M Bock
Journal:  Biochemistry       Date:  1965-07       Impact factor: 3.162

9.  Use of formaldehyde fixation for studies of ribonucleoprotein particles by caesium chloride density-gradient centrifugation.

Authors:  A S Spirin; N V Belitsina; M I Lerman
Journal:  J Mol Biol       Date:  1965-12       Impact factor: 5.469

10.  Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components.

Authors:  R P Perry; D E Kelley
Journal:  J Mol Biol       Date:  1966-04       Impact factor: 5.469
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  9 in total

1.  Differences between newly formed and recycled free small ribosome subunits in liver cytoplasm.

Authors:  R H Hinton; B M Mullock
Journal:  Biochem J       Date:  1976-07-15       Impact factor: 3.857

2.  Phosphorylation of ribosomal protein S6 in suspension cultured HeLa cells.

Authors:  S M Lastick; P J Nielsen; E H McConkey
Journal:  Mol Gen Genet       Date:  1977-04-29

3.  Proteomic analysis of ribosomes: translational control of mRNA populations by glycogen synthase GYS1.

Authors:  Gabriele Fuchs; Camille Diges; Lori A Kohlstaedt; Karen A Wehner; Peter Sarnow
Journal:  J Mol Biol       Date:  2011-05-05       Impact factor: 5.469

4.  Characterisation of ribosomal proteins from HeLa and Krebs II mouse ascites tumor cells by different two-dimensional polyacrylamide gel electrophoresis techniques.

Authors:  O G Issinger; H Beier
Journal:  Mol Gen Genet       Date:  1978-04-17

5.  Mitochondrial and cytoplasmic ribosomes from mammalian tissues. Further characterization of ribosomal subunits and validity of buoyant-density methods for determination of the chemical composition and partial specific volume of ribonucleoprotein particles.

Authors:  A Sacchi; U Ferrini; P Londei; P Cammarano; N Maraldi
Journal:  Biochem J       Date:  1977-11-15       Impact factor: 3.857

6.  Increased transcription and decreased degradation control and recovery of liver ribosomes after a period of protein starvation.

Authors:  R D Conde; M T Franze-Fernández
Journal:  Biochem J       Date:  1980-12-15       Impact factor: 3.857

7.  Messenger ribonucleoprotein complexes isolated by oligodeoxythymidylate-cellulose chromatography from Neurospora crassa polysomes.

Authors:  P E Mirkes
Journal:  J Bacteriol       Date:  1977-07       Impact factor: 3.490

8.  Heat shock induces rapid dephosphorylation of a ribosomal protein in Drosophila.

Authors:  C V Glover
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

9.  Mitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients.

Authors:  R J Lapolla; A M Lambowitz
Journal:  J Cell Biol       Date:  1982-10       Impact factor: 10.539
  9 in total

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