Urbanization and the microbial content of the north Saskatchewan River.

The effect of urbanization on the microbial content of the North Saskatchewan River was determined by following the changes in the numbers of total bacteria, total eosin methylene blue (EMB) plate count, and Escherichia coli as the river flowed from its glacial source, through parklands, and out into the prairies. Changes in physical parameters such as pH, temperature, salt concentration, and the amount and nature of the suspended material were also determined to evaluate their on the microbial parameters being measured. The level of all three microbial parameters studied slowly increased as the river flowed from its glacial source out into the prairies. The major effect of small hamlets, with or without sewage treatment facilities, appears to be to supply nutrients which supports the growth of the indigenous river flora but not E. coli. In contrast, the effect of a large urban center, with a population of approximately 500,000, which utilizes primary and secondary sewage processes in disposing of sewage, is to provide the nutrients and an inoculum of E. coli which results in a marked increase in the numbers of all three microbial groups studied. The effect of this urban center was still discernible 300 miles downstream. The river was also monitored for the presence of Salmonella sp. Only one positive isolation was achieved during this study, and this isolate was characterized as being Salmonella alachua.