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Literature DB >> 4565418

Location of the origin of transfer of the sex factor F.

Abstract

The location of the origin of transfer on the map of Flac has been determined by two different techniques. The first showed that no transfer cistrons were transferred early by a transposition Hfr strain with a known point of chromosomal integration into Flac, and the second used a series of transfer-deficient Hfr deletion strains to map a locus required in cis for chromosome transfer by an incoming Flac factor. Both techniques gave results consistent with a location for the origin of transfer between traJ and the phi(R) (11) locus.

Mesh：

Substances：
DNA, Bacterial
Lactose

Year: 1972 PMID： 4565418 PMCID： PMC251485 DOI： 10.1128/jb.112.2.773-778.1972

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

20 in total

Location of the origin of transfer of the sex factor F.

1. Characteristics of E. coli K12 strains carrying both an F prime and an R factor.

2. Chromosomal transfer mediated by de-repressed R factors in F- Escherichia coli K12.

Review 3. Current linkage map of Escherichia coli.

4. Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis.

5. The beginning of a genetic analysis of recombination proficiency.

6. Nonessential functions of bacteriophage lambda.

7. Chromosome transfer from F-lac+ strains of Escherichia coli K-12 mutant at recA, recB, or recC.

8. Strand selection during bacterial mating.

9. Asymmetric transfer of DNA strands in bacterial conjugation.

10. Chromosomal transfer from "recombination-deficient" strains of Escherichia coli K-12.

1. Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

2. cis-Dominant, transfer-deficient mutants of the Escherichia coli K-12 F sex factor.

3. Recombination and the Escherichia coli K-12 sex factor F.

4. Deletion analysis of the F plasmid oriT locus.

5. Transposon-mediated conjugational transmission of nonconjugative plasmids.

6. Intrinsic bends and integration host factor binding at F plasmid oriT.

7. Replication region fragments cloned from Flac+ are identical to EcoRI fragment f5 of F.

8. The control region of the F sex factor DNA transfer cistrons: physical mapping by deletion analysis.

9. Plasmid cointegrates of Flac and lambda prophage.

10. Assignment of tra cistrons to EcoRI fragments of F sex factor DNA.