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Literature DB >> 4558664

Phenylalanyl-tRNA synthetase and isoleucyl-tRNA Phe : a possible verification mechanism for aminoacyl-tRNA.

Abstract

The synthesis of isoleucyl-tRNA(Phe) (Escherichia coli) proceeds at an appreciable rate under normal in vitro conditions in the presence of isoleucyl-tRNA synthetase (EC 6.1.1.5) from E. coli. The misacylated product is shown here to be hydrolyzed by highly purified phenylalanyl-tRNA synthetase from E. coli, with release of isoleucine and active tRNA(Phe). Thus, phenylalanyl-tRNA synthetase possesses a previously unrecognized activity, which deacylates a mistakenly acylated tRNA(Phe); the enzyme is inactive toward correctly matched aminoacyl tRNAs. Such a mechanism could serve to verify aminoacyl-tRNAs, deacylating those that are misacylated. Thus, a common generalization needs to be modified: an amino acid is not necessarily committed to a given (incorrect) anticodon when it is incorporated into aminoacyl-tRNA. It may be possible to correct it thereafter.

Entities: Chemical Gene Species

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Year: 1972 PMID： 4558664 PMCID： PMC426831 DOI： 10.1073/pnas.69.7.1915

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

13 in total

1. DEMONSTRATION OF AN ALTERED AMINOACYL RIBONUCLEIC ACID SYNTHETASE IN A MUTANT OF ESCHERICHIA COLI.

Authors: W L FANGMAN; F C NEIDHARDT
Journal: J Biol Chem Date: 1964-06 Impact factor: 5.157

2. Purification and substrate specificity of a phenylalanine-activating enzyme from Escherichia coli 9723.

Authors: T W CONWAY; E M LANSFORD; W SHIVE
Journal: J Biol Chem Date: 1962-09 Impact factor: 5.157

3. On the role of soluble ribonucleic acid in coding for amino acids.

Authors: F CHAPEVILLE; F LIPMANN; G VON EHRENSTEIN; B WEISBLUM; W J RAY; S BENZER
Journal: Proc Natl Acad Sci U S A Date: 1962-06-15 Impact factor: 11.205

4. THE FUNCTION OF sRNA AS AMINO ACID ADAPTOR IN THE SYNTHESIS OF HEMOGLOBIN.

Authors: G Ehrenstein; B Weisblum; S Benzer
Journal: Proc Natl Acad Sci U S A Date: 1963-05 Impact factor: 11.205

5. A test of tRNA as amino acid adaptor in hemoglobin synthesis.

Authors: K B Jacobson
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. Purification and properties of isoleucyl ribonucleic acid synthetase from Escherichia coli.

Authors: A N Baldwin; P Berg
Journal: J Biol Chem Date: 1966-02-25 Impact factor: 5.157

8. Recognition of tRNA by isoleucyl-tRNA synthetase. Effect of substrates on the dynamics of tRNA-enzyme interaction.

Authors: M Yarus; P Berg
Journal: J Mol Biol Date: 1969-06-14 Impact factor: 5.469

9. Studies on the aminoacylation of valine- and alanine-specific transfer RNA of Escherichia coli by aminoacyl transfer RNA synthetases from Neurospora crassa and E. coli.

Authors: V Z Holten; K B Jacobson
Journal: Arch Biochem Biophys Date: 1969-01 Impact factor: 4.013

10. The yeast phenylalanyl-transfer RNA synthetase recognition site: the region adjacent to the dihydrouridine loop.

Authors: B Dudock; C DiPeri; K Scileppi; R Reszelbach
Journal: Proc Natl Acad Sci U S A Date: 1971-03 Impact factor: 11.205

24 in total

1. Participation by Drosophila transfer RNA in protein synthesis in an E. coli protein synthesizing system.

Authors: J A Kiger
Journal: Nucleic Acids Res Date: 1974-10 Impact factor: 16.971

2. Interstice mutations that block site-to-site translocation of a misactivated amino acid bound to a class I tRNA synthetase.

Authors: Anthony C Bishop; Kirk Beebe; Paul R Schimmel
Journal: Proc Natl Acad Sci U S A Date: 2003-01-06 Impact factor: 11.205

Phenylalanyl-tRNA synthetase and isoleucyl-tRNA Phe : a possible verification mechanism for aminoacyl-tRNA.

1. DEMONSTRATION OF AN ALTERED AMINOACYL RIBONUCLEIC ACID SYNTHETASE IN A MUTANT OF ESCHERICHIA COLI.

2. Purification and substrate specificity of a phenylalanine-activating enzyme from Escherichia coli 9723.

3. On the role of soluble ribonucleic acid in coding for amino acids.

4. THE FUNCTION OF sRNA AS AMINO ACID ADAPTOR IN THE SYNTHESIS OF HEMOGLOBIN.

5. A test of tRNA as amino acid adaptor in hemoglobin synthesis.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. Purification and properties of isoleucyl ribonucleic acid synthetase from Escherichia coli.

8. Recognition of tRNA by isoleucyl-tRNA synthetase. Effect of substrates on the dynamics of tRNA-enzyme interaction.

9. Studies on the aminoacylation of valine- and alanine-specific transfer RNA of Escherichia coli by aminoacyl transfer RNA synthetases from Neurospora crassa and E. coli.

10. The yeast phenylalanyl-transfer RNA synthetase recognition site: the region adjacent to the dihydrouridine loop.

1. Participation by Drosophila transfer RNA in protein synthesis in an E. coli protein synthesizing system.

2. Interstice mutations that block site-to-site translocation of a misactivated amino acid bound to a class I tRNA synthetase.

Review 3. Editing of errors in selection of amino acids for protein synthesis.

4. Proofreading in translation: dynamics of the double-sieve model.

5. An editing activity that prevents mistranslation and connection to disease.

Review 6. Aminoacyl tRNA synthetases and their connections to disease.

Review 7. Development of tRNA synthetases and connection to genetic code and disease.

8. Proofreading in vivo: editing of homocysteine by methionyl-tRNA synthetase in Escherichia coli.

9. On the evolution of accuracy and cost of proofreading tRNA aminoacylation.

10. Experimental evidence for kinetic proofreading in the aminoacylation of tRNA by synthetase.