Literature DB >> 4555634

Preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow ultracentrifugation.

D R Dubois, E C Cutchins, S Berman, J P Lowenthal, R L Timchak.   

Abstract

A method for the preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow density gradient ultracentrifugation is described. Both phases of the Henzerling strain of C. burneti were found in a zone between 53 and 65% (w/w) sucrose. Based on chemical assays, the Genetron zonal rickettsial suspensions were found to be as pure as the rickettsial suspensions which were prepared by the ether extraction method currently in use for producing Q fever vaccines for human use.

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Year:  1972        PMID： 4555634      PMCID： PMC380458          DOI： 10.1128/am.23.5.841-845.1972

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  13 in total

1.  Precise standardization of reagents for complement fixation.

Authors:  J F KENT; E H FIFE
Journal:  Am J Trop Med Hyg       Date:  1963-01       Impact factor: 2.345

2.  Variation in complement-fixing activity of Rickettsia burneti during egg adaptation.

Authors:  M G STOKER
Journal:  J Hyg (Lond)       Date:  1953-09

3.  METHOD FOR THE PRODUCTION OF A PURIFIED DRY Q FEVER VACCINE.

Authors:  S Berman; R B Gochenour; G Cole; J P Lowenthal; A S Benenson
Journal:  J Bacteriol       Date:  1961-05       Impact factor: 3.490

4.  Preparation of cholera vaccines by fluorocarbon extraction of cholera Vibrio suspensions.

Authors:  P L Altieri; S Berman; A Groffinger; J P Lowenthal
Journal:  Infect Immun       Date:  1970-04       Impact factor: 3.441

5.  A method of purifying Coxiella burnetii and other pathogenic Rickettsiae.

Authors:  R A ORMSBEE
Journal:  J Immunol       Date:  1962-01       Impact factor: 5.422

6.  Continuous-flow ultracentrifugation of canine distemper virus and infectious canine hepatitis virus.

Authors:  J A Elliott; W L Ryan
Journal:  Appl Microbiol       Date:  1970-11

7.  Isolation of polyoma virus and Semliki Forest virus from tissue culture fluid by continuous flow zonal ultracentrifugation.

Authors:  S M Fox; G D Birnie; E M Martin; J A Sonnabend
Journal:  J Gen Virol       Date:  1967-10       Impact factor: 3.891

8.  Purification of large quantities of influenza virus by density gradient centrifugation.

Authors:  C B Reimer; R S Baker; R M Van Frank; T E Newlin; G B Cline; N G Anderson
Journal:  J Virol       Date:  1967-12       Impact factor: 5.103

9.  Preparation of highly purified concentrates of Coxiella burneti.

Authors:  D S Spicer; A N DeSanctis; J M Beiler
Journal:  Proc Soc Exp Biol Med       Date:  1970-12

10.  Improved continuous flow centrifugation with banding.

Authors:  G B Cline; C E Nunley; N G Anderson
Journal:  Nature       Date:  1966-10-29       Impact factor: 49.962
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  2 in total

1.  Preparation of phase 1Q fever antigen suitable for vaccine use.

Authors:  D S Spicer; A N DeSanctis
Journal:  Appl Environ Microbiol       Date:  1976-07       Impact factor: 4.792

2.  Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation.

Authors:  E Weiss; J C Coolbaugh; J C Williams
Journal:  Appl Microbiol       Date:  1975-09
  2 in total

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