Literature DB >> 4320861

Continuous-flow ultracentrifugation of canine distemper virus and infectious canine hepatitis virus.

J A Elliott, W L Ryan.   

Abstract

The use of a Spinco L-4 zonal centrifuge with the B-XVI continuous-flow rotor for the purification of canine distemper and infectious canine hepatitis viruses is described. Up to 68 liters of virus was processed at one time. Infectious canine hepatitis virus was found to band at 39% sucrose and canine distemper virus banded between 32 and 48% sucrose. The virus was concentrated 10-fold, and the purity of the virus, as measured by protein concentration, was increased by 99%.

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Year:  1970        PMID： 4320861      PMCID： PMC377022          DOI： 10.1128/am.20.5.667-671.1970

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  6 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Use of zonal ultracentrifuge systems for biophysical studies of rhinoviruses.

Authors:  J L Gerin; W R Richter; J D Fenters; J C Holper
Journal:  J Virol       Date:  1968-09       Impact factor: 5.103

3.  Isolation of polyoma virus and Semliki Forest virus from tissue culture fluid by continuous flow zonal ultracentrifugation.

Authors:  S M Fox; G D Birnie; E M Martin; J A Sonnabend
Journal:  J Gen Virol       Date:  1967-10       Impact factor: 3.891

4.  Purification of large quantities of influenza virus by density gradient centrifugation.

Authors:  C B Reimer; R S Baker; R M Van Frank; T E Newlin; G B Cline; N G Anderson
Journal:  J Virol       Date:  1967-12       Impact factor: 5.103

5.  Improved continuous flow centrifugation with banding.

Authors:  G B Cline; C E Nunley; N G Anderson
Journal:  Nature       Date:  1966-10-29       Impact factor: 49.962

6.  Continuous-flow centrifugation combined with isopycnic banding: rotors B-8 and B-IX.

Authors:  N G Anderson; H P Barringer; J W Amburgey; G B Cline; C E Nunley; A S Berman
Journal:  Natl Cancer Inst Monogr       Date:  1966-06
  6 in total
  3 in total

1.  Determination of the buoyant density of canine distemper virus by radioassay.

Authors:  A M Armitage; H J Cornwell; N G Wright; A R Weir
Journal:  Arch Virol       Date:  1975       Impact factor: 2.574

2.  Preparation of purified suspensions of Coxiella burneti by Genetron extraction followed by continuous-flow ultracentrifugation.

Authors:  D R Dubois; E C Cutchins; S Berman; J P Lowenthal; R L Timchak
Journal:  Appl Microbiol       Date:  1972-05

3.  Buoyant density of canine distemper virus.

Authors:  L A Phillips; R H Bussell
Journal:  Arch Gesamte Virusforsch       Date:  1973
  3 in total

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