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Literature DB >> 4544010

Differential metabolism of large and small poly(A) sequences in the heterogeneous nuclear RNA of HeLa cells.

Abstract

The heterogeneous RNA of the HeLa cell nucleus contains short internal poly(A) sequences which, in contrast to the longer poly(A) sequences at the 3' ends, are not found in messenger RNA of the cytoplasm. A distinct origin for each of these homologous sequences is evident when the effects of actinomycin D and 3'-deoxyadenosine on their biosynthesis are compared. The shorter poly(A) appears to be transcribed, while the longer one does not. A kinetic analysis of the metabolism of each type of poly(A) sequence within different size classes of HnRNA after treatment of the cells with 3'-deoxyadenosine reveals distinctive distribution and metabolism of these sequences and also provides insight into mechanisms proposed for the generation of messenger RNA from these large RNA molecules in the nucleus.

Entities: Chemical

Mesh：

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Year: 1974 PMID： 4544010 PMCID： PMC387965 DOI： 10.1073/pnas.71.1.200

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Basis of actinomycin action. I. DNA binding and inhibition of RNA-polymerase synthetic reactions by actinomycin.

Authors: I H GOLDBERG; M RABINOWITZ; E REICH
Journal: Proc Natl Acad Sci U S A Date: 1962-12-15 Impact factor: 11.205

2. Polyadenylic acid sequences in yeast messenger ribonucleic acid.

Authors: C S McLaughlin; J R Warner; M Edmonds; H Nakazato; M H Vaughan
Journal: J Biol Chem Date: 1973-02-25 Impact factor: 5.157

3. Biogenesis of mRNA: genetic regulation in mammalian cells.

Authors: J E Darnell; W R Jelinek; G R Molloy
Journal: Science Date: 1973-09-28 Impact factor: 47.728

4. Localization of the polyadenylate sequences in messenger ribonucleic acid and in the heterogeneous nuclear ribonucleic acid of HeLa cells.

Authors: H Nakazato; D W Kopp; M Edmonds
Journal: J Biol Chem Date: 1973-02-25 Impact factor: 5.157

5. Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNA.

Authors: W Jelinek; M Adesnik; M Salditt; D Sheiness; R Wall; G Molloy; L Philipson; J E Darnell
Journal: J Mol Biol Date: 1973-04-15 Impact factor: 5.469

6. Occurrence of uridylate-rich oligonucleotide regions in heterogeneous nuclear RNA of HeLa cells.

Authors: G R Molloy; W L Thomas; J E Darnell
Journal: Proc Natl Acad Sci U S A Date: 1972-12 Impact factor: 11.205

7. Analysis of long pyrimidine polynucleotides in HeLa cell nuclear DNA: absence of polydeoxythymidylate.

Authors: H C Birnboim; R E Mitchel; N A Straus
Journal: Proc Natl Acad Sci U S A Date: 1973-08 Impact factor: 11.205

8. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.

Authors: M Edmonds; M H Vaughan; H Nakazato
Journal: Proc Natl Acad Sci U S A Date: 1971-06 Impact factor: 11.205

4. Nucleo-cytoplasmic relationships of high-molecular-weight ribonucleic acid, including polyadenylated species, in the developing rat brain.

Authors: W Berthold; L Lim
Journal: Biochem J Date: 1976-02-15 Impact factor: 3.857

9. Amount and distribution of virus-specific sequences in giant RNA molecules isolated from polyoma-infected mouse kidney cells.

Authors: Z Lev; H Manor
Journal: J Virol Date: 1977-03 Impact factor: 5.103

10. Adenosine utilization in cordycepin-sensitive mutants of Saccharomyces cerevisiae.

Authors: J M Anderson; R Roth
Journal: J Bacteriol Date: 1976-11 Impact factor: 3.490

Differential metabolism of large and small poly(A) sequences in the heterogeneous nuclear RNA of HeLa cells.

1. Basis of actinomycin action. I. DNA binding and inhibition of RNA-polymerase synthetic reactions by actinomycin.

2. Polyadenylic acid sequences in yeast messenger ribonucleic acid.

3. Biogenesis of mRNA: genetic regulation in mammalian cells.

4. Localization of the polyadenylate sequences in messenger ribonucleic acid and in the heterogeneous nuclear ribonucleic acid of HeLa cells.

5. Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNA.

6. Occurrence of uridylate-rich oligonucleotide regions in heterogeneous nuclear RNA of HeLa cells.

7. Analysis of long pyrimidine polynucleotides in HeLa cell nuclear DNA: absence of polydeoxythymidylate.

8. Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.

9. The turnover of nuclear DNA-like RNA in HeLa cells.

10. The selective interruption of nucleolar RNA synthesis in HeLa cells by cordycepin.

1. Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.

2. Characterization of polyadenylate from the fungus Trichoderma viride.

3. Possible relationship of poly(A) shortening to mRNA turnover.

4. Nucleo-cytoplasmic relationships of high-molecular-weight ribonucleic acid, including polyadenylated species, in the developing rat brain.

5. Effect of 5-bromodeoxyuridine on heterogeneous nuclear RNA in rat hepatoma cells.

6. Oligouridylate stretches in heterogeneous nuclear RNA.

7. The occurrence and distribution of poly(a) ribonucleic Acid in soybean.

8. Structure of polyadenylic acid in the ribonucleic acid of Saccharomyces cerevisiae.

9. Amount and distribution of virus-specific sequences in giant RNA molecules isolated from polyoma-infected mouse kidney cells.

10. Adenosine utilization in cordycepin-sensitive mutants of Saccharomyces cerevisiae.