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Literature DB >> 4530315

Use of eukaryotic native small ribosomal subunits for the translation of globin messenger RNA.

Abstract

A highly active in vitro system for the translation of globin mRNA, resulting in more than 10 rounds of translation, is described. The reconstituted system consists of native small ribosomal subunits of rabbit reticulocytes (as a source of initiation factors as well as small ribosomal subunits), large subunits derived from rat liver polysomes by the puromycin-KCl procedure, and a pH 5 fraction obtained from a Krebs ascites cell high speed supernatant. In this system no differences were found between globin messenger ribonucleoprotein and globin mRNA.

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Year: 1974 PMID： 4530315 PMCID： PMC433788 DOI： 10.1073/pnas.71.9.3435

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. Isolation of protein initiation factors from 30S ribosomal subunits.

Authors: R Parenti-Rosina; A Eisenstadt; J M Eisenstadt
Journal: Nature Date: 1969-01-25 Impact factor: 49.962

2. The role of the native subribosomal particles of Escherichia coli in polypeptide chain initiation.

Authors: J M Eisenstadt; G Brawerman
Journal: Proc Natl Acad Sci U S A Date: 1967-10 Impact factor: 11.205

3. A complex between met-tRNA F and native 40S subunits in reticulocyte lysates and its disappearance during incubation with double-stranded RNA.

Authors: C Darnbrough; T Hunt; R J Jackson
Journal: Biochem Biophys Res Commun Date: 1972-09-26 Impact factor: 3.575

4. Control of globin synthesis: the role of heme.

Authors: T Hunt; G Vanderhoff; I M London
Journal: J Mol Biol Date: 1972-05-28 Impact factor: 5.469

5. Protein tightly bound to globin mRNA.

Authors: G Blobel
Journal: Biochem Biophys Res Commun Date: 1972-04-14 Impact factor: 3.575

6. Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.

Authors: A K Falvey; T Staehelin
Journal: J Mol Biol Date: 1970-10-14 Impact factor: 5.469

7. Dissociation of mammalian polyribosomes into subunits by puromycin.

Authors: G Blobel; D Sabatini
Journal: Proc Natl Acad Sci U S A Date: 1971-02 Impact factor: 11.205

8. Release, identification, and isolation of messenger RNA from mammalian ribosomes.

Authors: G Blobel
Journal: Proc Natl Acad Sci U S A Date: 1971-04 Impact factor: 11.205

9. The ribosome cycle in a reconstituted cell-free system from reticulocytes.

Authors: S D Adamson; G A Howard; E Herbert
Journal: Cold Spring Harb Symp Quant Biol Date: 1969

10. Initiation of haemoglobin polypeptide chains.

Authors: J O Bishop
Journal: Biochim Biophys Acta Date: 1966-04-18

11 in total

1. mRNP proteins, initiation factors and phosphorylation.

Authors: J M Egly; R Elkaim; M Pierre
Journal: Mol Biol Rep Date: 1979-05-31 Impact factor: 2.316

2. Nonribosomal proteins associated with eukaryotic native small ribosomal subunits.

Authors: C Freienstein; G Blobel
Journal: Proc Natl Acad Sci U S A Date: 1975-09 Impact factor: 11.205

3. Analysis of mRNA populations by cDNA.mRNA hybrid-mediated inhibition of cell-free protein synthesis.

Authors: N D Hastie; W A Held
Journal: Proc Natl Acad Sci U S A Date: 1978-03 Impact factor: 11.205

4. Localization of eukaryotic initiation factor 3 on native small ribosomal subunits.

Authors: I Emanuilov; D D Sabatini; J A Lake; C Freienstein
Journal: Proc Natl Acad Sci U S A Date: 1978-03 Impact factor: 11.205

5. Cross-linking of mRNA to initiation factor eIF-3, 24 kDa cap binding protein and ribosomal proteins S1, S3/3a, S6 and S11 within the 48S pre-initiation complex.

Authors: P Westermann; O Nygård
Journal: Nucleic Acids Res Date: 1984-12-11 Impact factor: 16.971

9. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.

Authors: G Blobel; B Dobberstein
Journal: J Cell Biol Date: 1975-12 Impact factor: 10.539

10. Mechanism of compartmentation of secretory proteins: transport of exocrine pancreatic proteins across the microsomal membrane.

Authors: G Scheele; R Jacoby; T Carne
Journal: J Cell Biol Date: 1980-12 Impact factor: 10.539