Literature DB >> 4525299

Characterization of a protein species isolated from HeLa cell cytoplasm by affinity chromatography on polyadenylate-sepharose.

J M Blanchard, C Brissac, P Jeanteur.   

Abstract

Chromatography of different soluble extracts from HeLa cells on poly(A)-Sepharose columns has allowed the isolation of a protein fraction eluted by 0.2 M NaCl and localized predominantly in the cytoplasmic supernatant and in the 0.5 M KCl ribosomal wash. This fraction is present in large amounts (around 3% of total cytosolic proteins) and appears to contain a major protein species that is acidic on electrofocusing (pI around 4.5) and phosphorylated. It runs on glycerol gradients and Sephadex G-200 chromatography close to the aldolase marker (158,000 daltons) and dissociates into apparently identical subunits of 38,000 +/- 2,000 daltons on sodium dodecyl sulfate-acrylamide gels, suggesting a tetrameric structure.

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Year:  1974        PMID： 4525299      PMCID： PMC388346          DOI： 10.1073/pnas.71.5.1882

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  35 in total

1.  The effect of HeLa cell cytoplasm on the rate of sedimentation of RNA.

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Journal:  Proc Natl Acad Sci U S A       Date:  1966-09       Impact factor: 11.205

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10.  Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin.

Authors:  S Penman; M Rosbash; M Penman
Journal:  Proc Natl Acad Sci U S A       Date:  1970-12       Impact factor: 11.205
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  10 in total

1.  Interaction of poly(A) and mRNA with eukaryotic initiator met-tRNA-f binding factor: identification of this activity on reticulocyte ribonucleic acid protein particles.

Authors:  J G Hellerman; D A Shafritz
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

2.  Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of Artemia salina.

Authors:  H Slegers; E De Herdt; M Kondo
Journal:  Mol Biol Rep       Date:  1979-05-31       Impact factor: 2.316

3.  Extreme instability of myc mRNA in normal and transformed human cells.

Authors:  C Dani; J M Blanchard; M Piechaczyk; S El Sabouty; L Marty; P Jeanteur
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

4.  Messenger ribonucleoprotein complexes of cryptobiotic embryos of Artemia salina.

Authors:  H Slegers; M Kondo
Journal:  Nucleic Acids Res       Date:  1977-03       Impact factor: 16.971

5.  Preparation of poly (A)-binding proteins from endoplasmic reticulum-containing subfractions of rat liver cells and their use in mRNA purification.

Authors:  B Axelsson; R Ohlsson; A Deutsch; B Jergil
Journal:  Mol Cell Biochem       Date:  1977-03-21       Impact factor: 3.396

6.  RNA-binding properties of a translational activator, the adenovirus L4 100-kilodalton protein.

Authors:  D Riley; S J Flint
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

7.  Developmental changes in the protein and ribonucleic acid components of rat brain messenger ribonucleic acid-protein particles isolated from free polyribosomes by oligo(dT)-cellulose chromatography.

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Journal:  Biochem J       Date:  1980-07-15       Impact factor: 3.857

8.  Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.

Authors:  M S Swanson; G Dreyfuss
Journal:  Mol Cell Biol       Date:  1988-05       Impact factor: 4.272

9.  Nuclear proteins. II. Similarity of nonhistone proteins in nuclear sap and chromatin, and essential absence of contractile proteins from mouse liver nuclei.

Authors:  D E Comings; D C Harris
Journal:  J Cell Biol       Date:  1976-08       Impact factor: 10.539

10.  Finding one's way in proteomics: a protein species nomenclature.

Authors:  Hartmut Schlüter; Rolf Apweiler; Hermann-Georg Holzhütter; Peter R Jungblut
Journal:  Chem Cent J       Date:  2009-09-09       Impact factor: 4.215
  10 in total

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