Literature DB >> 4515608

Isolation of messenger RNA coding for mouse heavy-chain immunoglobulin.

R H Stevens, A R Williamson.   

Abstract

A method for purification of the mRNA coding for heavy-chain protein of a mouse G(2a) myeloma (5563) is reported. Complete myeloma protein (H(2)L(2)) specifically binds to the heavy-chain mRNA, and the resultant RNA-protein complex is precipitated with antiserum against 5563 myeloma protein. Cytoplasmic RNA isolated by this method showed two bands of 6.0 x 10(5) and 3.1 x 10(5) molecular weight when analyzed by acrylamide gel electrophoresis. The RNA in the bands contained 1.5% and 8% A-rich regions, respectively. The RNA in each of the bands contained sequences for heavy-chain protein when translated in Xenopus laevis oocytes.

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Year:  1973        PMID： 4515608      PMCID： PMC433440          DOI： 10.1073/pnas.70.4.1127

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Heavy and light chains of a homogeneous immunoglobulin-G.

Authors:  Z L. Awdeh; A R. Williamson; B A. Askonas
Journal:  FEBS Lett       Date:  1969-11-29       Impact factor: 4.124

2.  THE GROWTH AND MAINTENANCE OF TISSUE-CELL CULTURES IN FREE GAS EXCHANGE WITH THE ATMOSPHERE.

Authors:  A LEIBOVITZ
Journal:  Am J Hyg       Date:  1963-09

3.  A possible precursor of immunoglobulin light chains.

Authors:  C Milstein; G G Brownlee; T M Harrison; M B Mathews
Journal:  Nat New Biol       Date:  1972-09-27

4.  Isolation of duck haemoglobin messenger RNA and its translation by rabbit reticulocyte cell free system.

Authors:  R E Pemberton; D Housman; H F Lodish; C Baglioni
Journal:  Nat New Biol       Date:  1972-01-26

5.  Biologically active messenger-RNA for hemoglobin.

Authors:  J B Lingrel; R E Lockard; R F Jones; H E Burr; J W Holder
Journal:  Ser Haematol       Date:  1971

6.  A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.

Authors:  S Y Lee; J Mendecki; G Brawerman
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205

7.  The isolation and DNA-RNA hybridization of messenger RNA for globin.

Authors:  R Williamson; M R Morrison
Journal:  Ser Haematol       Date:  1971

8.  Purification and properties of biologically active messenger RNA for a myeloma light chain.

Authors:  D Swan; H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205

9.  One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins.

Authors:  Z L Awdeh; A R Williamson; B A Askonas
Journal:  Biochem J       Date:  1970-01       Impact factor: 3.857

10.  RNA metabolism in HeLa cells at reduced temperature. I. Modified processing of 45S RNA.

Authors:  R H Stevens; H Amos
Journal:  J Cell Biol       Date:  1971-09       Impact factor: 10.539
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  7 in total

1.  Cleavage of adenovirus messenger RNA and of 28S and 18S ribosomal RNA by RNase III.

Authors:  H Westphal; R J Crouch
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

2.  A general method for isolation of individual poly-ribosomes and pure mRNAs by immunosorption technique.

Authors:  E V Sidorova; M G Trudolyubova; M I Lerman
Journal:  Mol Biol Rep       Date:  1974-09       Impact factor: 2.316

3.  Germ line basis for antibody diversity: immunoglobulin VH-and CH-gene frequencies measured by DNA-RNA hybridization.

Authors:  E Premkumar; M Shoyab; A R Williamson
Journal:  Proc Natl Acad Sci U S A       Date:  1974-01       Impact factor: 11.205

4.  The regulation of protein synthesis in heart muscle under normal conditions and in the adriamycin-cardiomyopathy.

Authors:  J Zähringer
Journal:  Klin Wochenschr       Date:  1981-12-01

Review 5.  Immunoglobulin formation in B lymphoid cells.

Authors:  B A Askonas
Journal:  J Clin Pathol Suppl (Assoc Clin Pathol)       Date:  1975

6.  Isolation and in vitro translation of delta-crystallin mRNA from embryonic chick lens fibers.

Authors:  P Zelenka; J Piatigorsky
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

7.  Isolation of mRNA coding for immunoglobulin heavy chain.

Authors:  R H Stevens; A R Williamson
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205
  7 in total

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