Literature DB >> 4509644

Stability of cytoplasmic messenger RNA in HeLa cels.

W Murphy, G Attardi.   

Abstract

The metabolic stability of pulse-labeled or long-term labeled messenger RNA (mRNA) from cytoplasmic free polysomes was measured in HeLa cells, with chase conditions that do not involve inhibitors of RNA synthesis, and chromatography on benzoylated-DEAE-cellulose or poly(T)-cellulose for isolation of mRNA. For these studies, a new chase technique was developed that allows analysis of the stability of mRNA labeled during a short [(3)H]uridine pulse. Pulse-labeled and long-term labeled mRNA were found to decay with an estimated average half-life of about 2 and 3 days, respectively, much longer than hitherto assumed.

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Year:  1973        PMID： 4509644      PMCID： PMC433196          DOI： 10.1073/pnas.70.1.115

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  THE STABILITY OF LIVER MESSENGER RNA.

Authors:  M REVEL; H H HIATT
Journal:  Proc Natl Acad Sci U S A       Date:  1964-05       Impact factor: 11.205

2.  Expression of the mitochondrial genome in HeLa cells. IX. Replication of mitochondrial DNA in relationship to cell cycle in HeLa cells.

Authors:  L Pica-Mattoccia; G Attardi
Journal:  J Mol Biol       Date:  1972-03-14       Impact factor: 5.469

3.  The isolation and purification of rapidly labeled polysome-bound ribonucleic acid on polythymidylate cellulose.

Authors:  H Nakazato; M Edmonds
Journal:  J Biol Chem       Date:  1972-05-25       Impact factor: 5.157

4.  Detection of an unstable RNA in chick fibroblasts after reduction of the UTP pool by glucosamine.

Authors:  C Scholtissek
Journal:  Eur J Biochem       Date:  1971-12

Review 5.  Messenger RNA.

Authors:  E P Geiduschek; R Haselkorn
Journal:  Annu Rev Biochem       Date:  1969       Impact factor: 23.643

6.  Purification of Escherichia coli pulse-labeled RNA by benzoylated DEAE-cellulose chromatography.

Authors:  J Sedat; A Lyon; R L Sinsheimer
Journal:  J Mol Biol       Date:  1969-09-28       Impact factor: 5.469

7.  Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger.

Authors:  T W Borun; M D Scharff; E Robbins
Journal:  Proc Natl Acad Sci U S A       Date:  1967-11       Impact factor: 11.205

8.  High molecular weight nonribosomal-type nuclear RNA and cytoplasmic messenger RNA in HeLa cells.

Authors:  J F Houssais; G Attardi
Journal:  Proc Natl Acad Sci U S A       Date:  1966-08       Impact factor: 11.205

9.  Nucleotide metabolism in tissue culture cells at low temperatures. I. Phosphorylation of nucleosides and deoxynucleosides in vivo.

Authors:  C Scholtissek
Journal:  Biochim Biophys Acta       Date:  1967-09-26

10.  Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.

Authors:  M Edmonds; M H Vaughan; H Nakazato
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205
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  19 in total

1.  Possible relationship of poly(A) shortening to mRNA turnover.

Authors:  D Sheiness; L Puckett; J E Darnell
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

2.  Intracellular distribution and sedimentation properties of virus-specific RNA in two clones of BHK cells transformed by polyoma virus.

Authors:  I H Maxwell
Journal:  J Virol       Date:  1976-05       Impact factor: 5.103

3.  Balbiani ring nucleotide sequences in cytoplasmic 75S RNA of Chironomus tentans salivary gland cells.

Authors:  B Lambert; J E Edström
Journal:  Mol Biol Rep       Date:  1974-12       Impact factor: 2.316

4.  Distribution of repetitive and nonrepetivite sequence transcripts in HeLa mRNA.

Authors:  W H Klein; W Murphy; G Attardi; R J Britten; E H Davidson
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

5.  Synthesis and turnover of mitochondrial ribonucleic acid in HeLa cells: the mature ribosomal and messenger ribonucleic acid species are metabolically unstable.

Authors:  R Gelfand; G Attardi
Journal:  Mol Cell Biol       Date:  1981-06       Impact factor: 4.272

Review 6.  mRNA stability in mammalian cells.

Authors:  J Ross
Journal:  Microbiol Rev       Date:  1995-09

7.  Onset of ribosome degradation during cessation of growth in BHK-21/C13 cells.

Authors:  W T Melvin; H M Keir
Journal:  Biochem J       Date:  1978-12-15       Impact factor: 3.857

8.  RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells.

Authors:  Y Aloni; M Shani; Y Reuveni
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

9.  Synthesis of phosphoenolpyruvate carboxykinase (guanosine triphosphate) by isolated liver polyribosomes.

Authors:  F J Ballard; M F Hopgood; L Reshef; S Tilghman; R W Hanson
Journal:  Biochem J       Date:  1974-11       Impact factor: 3.857

10.  Rapid loss of translatable messenger RNA of phosphoenolpyruvate carboxykinase during glucose repression in liver.

Authors:  S M Tilghman; R W Hanson; L Reshef; M F Hopgood; F J Ballard
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205
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