Literature DB >> 4525461

Distribution of repetitive and nonrepetivite sequence transcripts in HeLa mRNA.

W H Klein, W Murphy, G Attardi, R J Britten, E H Davidson.   

Abstract

Polyadenylated messenger RNA extracted from HeLa cells was hybridized with a mass excess of HeLa DNA. The kinetics of the hybridization reaction demonstrated that most of the messenger RNA is transcribed from nonrepetitive DNA. The amount of messenger RNA hybridized to DNA was measured both with and without prior RNase treatment. Comparison of the results indicates that within the limits of detection, HeLa messenger RNA does not contain repetitive sequence elements covalently linked to nonrepetitive sequence transcripts. However, a small fraction of the HeLa messenger RNA preparation is transcribed entirely from repetitive DNA sequences. This fraction represents about 6% of the total polyadenylated messenger RNA preparation.

Mesh：

Substances：

Year:  1974        PMID： 4525461      PMCID： PMC388325          DOI： 10.1073/pnas.71.5.1785

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Evidence for single copies of globin genes in the mouse genome.

Authors:  P R Harrison; A Hell; G D Birnie; J Paul
Journal:  Nature       Date:  1972-09-22       Impact factor: 49.962

2.  Reiteration frequency of haemoglobin genes in the duck.

Authors:  J O Bishop; R Pemberton; C Baglioni
Journal:  Nat New Biol       Date:  1972-02-23

3.  Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.

Authors:  R A Firtel; A Jacobson; H F Lodish
Journal:  Nat New Biol       Date:  1972-10-25

4.  Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde.

Authors:  H Boedtker
Journal:  J Mol Biol       Date:  1968-07-14       Impact factor: 5.469

5.  DNA-RNA hybridization in vast DNA excess.

Authors:  M Melli; C Whitfield; K V Rao; M Richardson; J O Bishop
Journal:  Nat New Biol       Date:  1971-05-05

6.  The genes for silk fibroin in Bombyx mori.

Authors:  Y Suzuki; L P Gage; D D Brown
Journal:  J Mol Biol       Date:  1972-10-14       Impact factor: 5.469

7.  The distribution of rapidly hybridizing RNA sequences in heterogeneous nuclear RNA and mRNA from HeLa cells.

Authors:  J E Darnell; R Balint
Journal:  J Cell Physiol       Date:  1970-12       Impact factor: 6.384

8.  Electron microscopic visualization of mitochondrial RNA-DNA hybrids.

Authors:  D Robberson; Y Aloni; G Attardi
Journal:  J Mol Biol       Date:  1971-01-28       Impact factor: 5.469

9.  Reiteration and clustering of DNA sequences complementary to histone messenger RNA.

Authors:  L H Kedes; M L Birnstiel
Journal:  Nat New Biol       Date:  1971-04-07

10.  Hybridization properties of DNA sequences directing the synthesis of messenger RNA and heterogeneous nuclear RNA.

Authors:  J R Greenberg; R P Perry
Journal:  J Cell Biol       Date:  1971-09       Impact factor: 10.539
View more
  20 in total

1.  Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.

Authors:  R W Pero; T Bryngelsson; C Bryngelsson; A Deutsch; A Nordén; A Norgren
Journal:  Nucleic Acids Res       Date:  1975-07       Impact factor: 16.971

2.  Evolutionary divergence and length of repetitive sequences in sea urchin DNA.

Authors:  R J Britten; D E Graham; F C Eden; D M Painchaud; E H Davidson
Journal:  J Mol Evol       Date:  1976-12-31       Impact factor: 2.395

3.  Comparison of nucleotide sequences in HeLa cell mRNA and hnRNA.

Authors:  N W Fraser; R H Burdon; R A Elton
Journal:  Nucleic Acids Res       Date:  1975-11       Impact factor: 16.971

4.  Direct demonstration of a complementarity between mRNA and double-stranded sequences of pre-mRNA.

Authors:  A P Ryskov; O V Tokarskaya; G P Georgiev
Journal:  Mol Biol Rep       Date:  1976-04       Impact factor: 2.316

5.  Functional shift from muscarinic to nicotinic cholinergic receptors involved in inositol trisphosphate and cyclic GMP accumulation during the primary culture of adrenal chromaffin cells.

Authors:  T Nakaki; N Sasakawa; S Yamamoto; R Kato
Journal:  Biochem J       Date:  1988-04-15       Impact factor: 3.857

6.  Purification and characterization of two transcribed repetitive DNA fractions from the pigeon genome.

Authors:  K G Gasaryan; E D Kuznetsova; V Z Tarantul; S A Sivak
Journal:  Chromosoma       Date:  1977-06-23       Impact factor: 4.316

7.  Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes.

Authors:  J J Yunis; M T Kuo; G F Saunders
Journal:  Chromosoma       Date:  1977-06-23       Impact factor: 4.316

8.  Hybridization properties of immunoglobulin mRNA: failure to detect covalently associated IgG mRNA transcripts of reiterated and unique mouse DNA.

Authors:  M K Legler; E P Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

9.  mRNA in human cells contains sequences complementary to the Alu family of repeated DNA.

Authors:  B Calabretta; D L Robberson; A L Maizel; G F Saunders
Journal:  Proc Natl Acad Sci U S A       Date:  1981-10       Impact factor: 11.205

10.  The expression of a plant genome in hnRNA and mRNA.

Authors:  M Kiper; D Bartels; F Herzfeld; G Richter
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.