Literature DB >> 450707

The effect of aphidicolin on DNA synthesis in isolated HeLa cell nuclei.

E Wist, H Prydz.   

Abstract

The effect of the inhibitor aphidicolin on DNA synthesis in isolated nuclei from HeLa cells and on the activities of partially purified DNA polymerases has been tested. Aphidicolin inhibited DNA synthesis and DNA polymerase alpha very efficiently whereas DNA polymerases beta and gamma were insensitive to the drug. The results indicate that DNA polymerase alpha is the polymerase active during elongation as well as in the gapfilling process of discontinuous DNA synthesis.

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Year:  1979        PMID： 450707      PMCID： PMC327792          DOI： 10.1093/nar/6.4.1583

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

2.  Role of deoxyribonucleic acid polymerase beta in nuclear deoxyribonucleic acid synthesis.

Authors:  T R Butt; W M Wood; R L Adams
Journal:  Biochem Soc Trans       Date:  1976       Impact factor: 5.407

3.  Okazaki pieces grow opposite to the replication fork direction during simian virus 40 DNA replication.

Authors:  G Kaufmann; R Bar-Shavit; M L DePamphilis
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

4.  Involvement of DNA polymerase alpha in simian virus 40 DNA replication.

Authors:  H J Edenberg; S Anderson; M L DePamphilis
Journal:  J Biol Chem       Date:  1978-05-10       Impact factor: 5.157

5.  Aphidicolin: a specific inhibitor of DNA polymerases in the cytosol of rat liver.

Authors:  M Ohashi; T Taguchi; S Ikegami
Journal:  Biochem Biophys Res Commun       Date:  1978-06-29       Impact factor: 3.575

6.  Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha.

Authors:  S Ikegami; T Taguchi; M Ohashi; M Oguro; H Nagano; Y Mano
Journal:  Nature       Date:  1978-10-05       Impact factor: 49.962

7.  Involvement of deoxyribonucleic acid polymerase beta in nuclear deoxyribonucleic acid synthesis.

Authors:  T R Butt; W M Wood; E L McKay; R L Adams
Journal:  Biochem J       Date:  1978-07-01       Impact factor: 3.857

8.  Effect of 2',3'-dideoxythymidine-5'-triphosphate on HeLa cell in vitro DNA synthesis: evidence that DNA polymerase alpha is the only polymerase required for cellular DNA replication.

Authors:  M A Waqar; M J Evans; J A Huberman
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971

9.  Preservation by glycerol of DNA synthetic capacity of isolated HeLa S(3) cell nuclei during long term storage at low temperature.

Authors:  E Wist; H Krokan
Journal:  Exp Cell Res       Date:  1978-10-15       Impact factor: 3.905

10.  Role of DNA polymerase gamma in adenovirus DNA replication.

Authors:  P C van der Vliet; M M Kwant
Journal:  Nature       Date:  1978-11-30       Impact factor: 49.962
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  22 in total

1.  Further characterization of a cell-free system for measuring replicative and repair DNA synthesis with cultured human fibroblasts and evidence for the involvement of DNA polymerase alpha in DNA repair.

Authors:  G Ciarrocchi; J G Jose; S Linn
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971

2.  Complementation of the temperature-sensitive defect in H5ts125 adenovirus DNA replication in vitro.

Authors:  L M Kaplan; H Ariga; J Hurwitz; M S Horwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1979-11       Impact factor: 11.205

3.  The presence of intact mitochondrial DNA in HeLa cell nuclei.

Authors:  T Kristensen; H Prydz
Journal:  Nucleic Acids Res       Date:  1986-03-25       Impact factor: 16.971

4.  Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.

Authors:  B W Stillman; Y Gluzman
Journal:  Mol Cell Biol       Date:  1985-08       Impact factor: 4.272

5.  Butylanilinouracil: a selective inhibitor of HeLa cell DNA synthesis and HeLa cell DNA polymerase alpha.

Authors:  G E Wright; E F Baril; N C Brown
Journal:  Nucleic Acids Res       Date:  1980-01-11       Impact factor: 16.971

6.  The effect of aphidicolin on adenovirus DNA synthesis.

Authors:  M Longiaru; J E Ikeda; Z Jarkovsky; S B Horwitz; M S Horwitz
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

7.  Impact of 2-bromo-5,6-dichloro-1-beta-D-ribofuranosyl benzimidazole riboside and inhibitors of DNA, RNA, and protein synthesis on human cytomegalovirus genome maturation.

Authors:  Michael A McVoy; Daniel E Nixon
Journal:  J Virol       Date:  2005-09       Impact factor: 5.103

8.  Elongation of primed DNA templates by eukaryotic DNA polymerases.

Authors:  J E Ikeda; M Longiaru; M S Horwitz; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1980-10       Impact factor: 11.205

9.  Interphase cytofission maintains genomic integrity of human cells after failed cytokinesis.

Authors:  Alka Choudhary; Robert F Lera; Melissa L Martowicz; Kim Oxendine; Jennifer J Laffin; Beth A Weaver; Mark E Burkard
Journal:  Proc Natl Acad Sci U S A       Date:  2013-07-22       Impact factor: 11.205

10.  Inhibition of DNA polymerase alpha by aphidicolin derivatives.

Authors:  L Arabshahi; N Brown; N Khan; G Wright
Journal:  Nucleic Acids Res       Date:  1988-06-10       Impact factor: 16.971
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