Literature DB >> 209411

Okazaki pieces grow opposite to the replication fork direction during simian virus 40 DNA replication.

G Kaufmann, R Bar-Shavit, M L DePamphilis.   

Abstract

Simian virus 40 replicating DNA was pulse labeled with alpha-32P-dATP using an acellular DNA replication system. Nascent DNA chains of less than 200 nucleotides (Okazaki pieces) were then isolated from the denatured replicating DNA by electrosieving through a polyacrylamide gel column. The purified Okazaki pieces were hybridized to separated strands of Bg1(1)+Hpa1 simian virus 40 DNA restriction fragments immobilized on nitrocellulose filters. Only strands with polarity of the DNA replication fork direction hybridized with Okazaki pieces. Hence, Okazaki pieces in simian virus 40 are synthesized against the DNA replication fork direction.

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Year:  1978        PMID： 209411      PMCID： PMC342183          DOI： 10.1093/nar/5.7.2535

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

Review 1.  The genome of simian virus 40.

Authors:  T J Kelly; D Nathans
Journal:  Adv Virus Res       Date:  1977       Impact factor: 9.937

2.  Strandedness of newly synthesized short pieces of polyoma DNA from isolated nuclei.

Authors:  P J Flory
Journal:  Nucleic Acids Res       Date:  1977       Impact factor: 16.971

3.  Cooperative alignment of nu bodies during chromosome replication in the presence of cycloheximide.

Authors:  H Weintraub
Journal:  Cell       Date:  1976-11       Impact factor: 41.582

4.  Studies on the mode of segregation of histone nu bodies during replication in HeLa cells.

Authors:  R L Seale
Journal:  Cell       Date:  1976-11       Impact factor: 41.582

5.  Asymmetric Okazaki piece synthesis during replication of simian virus 40 DNA in vivo.

Authors:  D Perlman; J A Huberman
Journal:  Cell       Date:  1977-12       Impact factor: 41.582

6.  In vitro polyoma DNA synthesis: asymmetry of short DNA chains.

Authors:  T Hunter; B Francke; L Bacheler
Journal:  Cell       Date:  1977-12       Impact factor: 41.582

7.  RNA primers in Simian virus 40 DNA replication. II. Distribution of 5' terminal oligoribonucleotides in nascent DNA.

Authors:  G Kaufmann; S Anderson; M L DePamphilis
Journal:  J Mol Biol       Date:  1977-11-05       Impact factor: 5.469

8.  Excision repair of uracil incorporated in DNA as a result of a defect in dUTPase.

Authors:  B K Tye; I R Lehman
Journal:  J Mol Biol       Date:  1977-12-05       Impact factor: 5.469

9.  Replication of polyoma DNA in isolated nuclei. 3. The nucleotide sequence at the RNA-DNA junction of nascent strands.

Authors:  V Pigiet; R Eliasson; P Reichard
Journal:  J Mol Biol       Date:  1974-03-25       Impact factor: 5.469

10.  Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences.

Authors:  Y Aloni; R Dhar; O Laub; M Horowitz; G Khoury
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205
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  15 in total

Review 1.  Chromatin replication revealed by studies of animal cells and papovaviruses (simian virus 40 and polyoma virus).

Authors:  C Crémisi
Journal:  Microbiol Rev       Date:  1979-09

2.  Polyoma virus DNA replication is semi-discontinuous.

Authors:  E A Hendrickson; C E Fritze; W R Folk; M L DePamphilis
Journal:  Nucleic Acids Res       Date:  1987-08-25       Impact factor: 16.971

3.  Discontinuous synthesis of both strands at the growing fork during polyoma DNA replication in vitro.

Authors:  M Närkhammar-Meuth; R Eliasson; G Magnusson
Journal:  J Virol       Date:  1981-07       Impact factor: 5.103

4.  Both strands of polyoma DNA are replicated discontinuously with ribonucleotide primers in vivo.

Authors:  M Närkhammar-Meuth; J Kowalski; D T Denhardt
Journal:  J Virol       Date:  1981-07       Impact factor: 5.103

5.  A cloned immunoglobulin cDNA fragment enhances transposition of IS elements into recombinant plasmids.

Authors:  O Amster; D Salomon; A Zamir
Journal:  Nucleic Acids Res       Date:  1982-08-11       Impact factor: 16.971

6.  The effect of aphidicolin on DNA synthesis in isolated HeLa cell nuclei.

Authors:  E Wist; H Prydz
Journal:  Nucleic Acids Res       Date:  1979-04       Impact factor: 16.971

7.  Escherichia coli RNA polymerase in vitro mimics simian virus 40 in vivo transcription when the template is viral nucleoprotein.

Authors:  E B Jakobovits; S Saragosti; M Yaniv; Y Aloni
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

8.  Distribution of replicating simian virus 40 DNA in intact cells and its maturation in isolated nuclei.

Authors:  D P Tapper; S Anderson; M L DePamphilis
Journal:  J Virol       Date:  1982-03       Impact factor: 5.103

9.  Nucleosome segregation at a defined mammalian chromosomal site.

Authors:  D J Roufa; M A Marchionni
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

10.  Mouse DNA polymerase alpha-primase terminates and reinitiates DNA synthesis 2-14 nucleotides upstream of C2A1-2(C2-3/T2) sequences on a minute virus of mice DNA template.

Authors:  E A Faust; R Nagy; S K Davey
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205
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