Literature DB >> 4504371

Separation and translation of the mRNAs coding for and chains of rabbit globin.

G F Temple, D E Housman.   

Abstract

A method is presented to separate the mRNAs coding for alpha and beta globins of rabbit reticulocytes. 10S RNA was extracted from the light and heavy polysomes created by incubation of reticulocytes with L-O-methylthreonine, and assayed for mRNA activity in an ascites cell-free extract. Tryptic digests of the in vitro products demonstrated that the heavy polysomes yielded beta globin mRNA at least 90% free of alpha mRNA activity, and that the light polysomes yielded alpha mRNA at least 70% free of beta mRNA activity.

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Year:  1972        PMID： 4504371      PMCID： PMC426751          DOI： 10.1073/pnas.69.6.1574

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Regulation of in vitro translation of bacteriophage f2 RNA.

Authors:  H F Lodish; H D Robertson
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

2.  Initiation of haemoglobin synthesis by methionyl-tRNA.

Authors:  D Housman; M Jacobs-Lorena; U L Rajbhandary; H F Lodish
Journal:  Nature       Date:  1970-08-29       Impact factor: 49.962

3.  Control of translation of T4 phage: altered ribosome binding at R17 initiation sites.

Authors:  J A Steitz; S K Dube; P S Rudland
Journal:  Nature       Date:  1970-05-30       Impact factor: 49.962

4.  Untranslated nucleotide sequence at the 5'-end of R17 bacteriophage RNA.

Authors:  J M Adams; S Cory
Journal:  Nature       Date:  1970-08-08       Impact factor: 49.962

5.  The characterization of separated alpha and beta-chain polyribosomes in rabbit reticulocytes.

Authors:  H H Kazazian; M L Freedman
Journal:  J Biol Chem       Date:  1968-12-25       Impact factor: 5.157

6.  The synthesis of mouse hemoglobin beta-chains in a rabbit reticulocyte cell-free system programmed with mouse reticulocyte 9S RNA.

Authors:  R E Lockard; J B Lingrel
Journal:  Biochem Biophys Res Commun       Date:  1969-10-08       Impact factor: 3.575

7.  Translational control in hemoglobin syntheskis.

Authors:  M Rabinovitz; M L Freedman; J M Fisher; C R Maxwell
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

8.  Polyribosome dissociation and formation in intact reticulocytes with conservation of messenger ribonucleic acid.

Authors:  P A Marks; E R Burka; F M Conconi; W Perl; R A Rifkind
Journal:  Proc Natl Acad Sci U S A       Date:  1965-06       Impact factor: 11.205

9.  Characterization of macromolecules by constant velocity sedimentation.

Authors:  H Noll
Journal:  Nature       Date:  1967-07-22       Impact factor: 49.962

10.  The mechanism of sodium fluoride and cycloheximide inhibition of hemoglobin biosynthesis in the cell-free reticulocyte system.

Authors:  S Y Lin; R D Mosteller; B Hardesty
Journal:  J Mol Biol       Date:  1966-10-28       Impact factor: 5.469
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  6 in total

1.  Effect of hemin on the synthesis of hemoglobin and other proteins in mammalian cells.

Authors:  Y Beuzard; R Rodvien; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1973-04       Impact factor: 11.205

2.  A specific dimerization of rabbit beta-globin messenger ribonucleic acid.

Authors:  V A Mezl; J A Hunt
Journal:  Biochem J       Date:  1978-10-01       Impact factor: 3.857

3.  The effects of hemin and double-stranded RNA on alpha and beta globin synthesis in reticulocyte and Krebs II ascites cell-free systems and the relationship of these effects to an initiation factor preparation.

Authors:  Y Beuzard; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1974-07       Impact factor: 11.205

4.  Quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes.

Authors:  D Housman; B G Forget; A Skoultchi; E J Benz
Journal:  Proc Natl Acad Sci U S A       Date:  1973-06       Impact factor: 11.205

5.  Decreased globin messenger RNA in thalassemia detected by molecular hybridization.

Authors:  D L Kacian; R Gambino; L W Dow; E Grossbard; C Natta; F Ramirez; S Spiegelman; P A Marks; A Bank
Journal:  Proc Natl Acad Sci U S A       Date:  1973-06       Impact factor: 11.205

6.  An electrophoretically silent polymorphism for the beta chains of rabbit hemoglobin and associated polyribosome patterns.

Authors:  M D Garrick; J Bricker; L M Garrick
Journal:  Genetics       Date:  1974-01       Impact factor: 4.562
  6 in total

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