Literature DB >> 4478065

The effects of two disulphides on cholinesterase activity in the spectrophotometric assay.

K B Augustinsson, H Eriksson.   

Abstract

1. 5,5'-Dithiobis-(2-nitrobenzoate) did not influence serum cholinesterase activity, whereas 2,2'-dithiodipyridine had an inhibitory effect. 2. The lowering of the molar extinction coefficients observed in the presence of physostigmine may be a result of a reaction between thiolate ions with carbamate moieties. 3. The use of 5,5'-dithiobis-(2-nitrobenzoate) is still recommended in investigations, especially where the quantitative aspects are significant.

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Year:  1974        PMID： 4478065      PMCID： PMC1166258          DOI： 10.1042/bj1390123

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  5 in total

1.  A new and rapid colorimetric determination of acetylcholinesterase activity.

Authors:  G L ELLMAN; K D COURTNEY; V ANDRES; R M FEATHER-STONE
Journal:  Biochem Pharmacol       Date:  1961-07       Impact factor: 5.858

2.  Assay methods for cholinesterases.

Authors:  K B AUGUSTINSSON
Journal:  Methods Biochem Anal       Date:  1957

3.  Aromatic thiol esters as substrates in quantitative esterase determinations.

Authors:  K B Augustinsson; B Axenfors
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

4.  Determination of sulfhydryl groups with 2,2'- or 4,4'-dithiodipyridine.

Authors:  D R Grassetti; J F Murray
Journal:  Arch Biochem Biophys       Date:  1967-03       Impact factor: 4.013

5.  The modification of cholinesterase activity by 5,5'-dithiobis-(2-nitrobenzoic acid) included in the coupled spectrophotometric assay. Evidence for a non-catalytic substrate-binding site.

Authors:  C Brownson; D C Watts
Journal:  Biochem J       Date:  1973-02       Impact factor: 3.857
  5 in total
  4 in total

1.  Mitochondrial dysfunction may explain the cardiomyopathy of chronic iron overload.

Authors:  Xueshan Gao; Mingwei Qian; Jian Li Campian; James Marshall; Zhanxiang Zhou; Andrew M Roberts; Y James Kang; Sumanth D Prabhu; Xiao-Feng Sun; John W Eaton
Journal:  Free Radic Biol Med       Date:  2010-05-05       Impact factor: 7.376

2.  The effect of some amine oxides and disulphide compounds on the activity of acetylcholinesterase.

Authors:  R M Dawson
Journal:  Biochem J       Date:  1975-07       Impact factor: 3.857

3.  A steady-state kinetic model of butyrylcholinesterase from horse plasma.

Authors:  K B Augustinsson; T Bartfai; B Mannervik
Journal:  Biochem J       Date:  1974-09       Impact factor: 3.857

4.  Cholinesterase levels in blood plasma and erythrocytes from calves, normal delivering cows and cows suffering from parturient paresis.

Authors:  K Forslund; C Björkman; M Abrahamsson
Journal:  Acta Vet Scand       Date:  1983       Impact factor: 1.695
  4 in total

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