Literature DB >> 4391800

Competent Diplococcus pneumoniae accept both single- and double-stranded deoxyribonucleic acid.

R Miao, W R Guild.   

Abstract

The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normally prepared competent cells of this species. Their efficiency is about 0.5% that of native DNA of comparable size.

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Year:  1970        PMID： 4391800      PMCID： PMC284915          DOI： 10.1128/jb.101.2.361-364.1970

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  19 in total

1.  THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE).

Authors:  I R LEHMAN; A L NUSSBAUM
Journal:  J Biol Chem       Date:  1964-08       Impact factor: 5.157

2.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

3.  The bouyant behavior of viral and bacterial DNA in alkaline CsCl.

Authors:  J VINOGRAD; J MORRIS; N DAVIDSON; W F DOVE
Journal:  Proc Natl Acad Sci U S A       Date:  1963-01-15       Impact factor: 11.205

4.  The native, denatured and renatured states of deoxyribonucleic acid.

Authors:  J Eigner; P Doty
Journal:  J Mol Biol       Date:  1965-07       Impact factor: 5.469

5.  Number of transformable units per cell in Diplococcus pneumoniae.

Authors:  R D Porter; W R Guild
Journal:  J Bacteriol       Date:  1969-03       Impact factor: 3.490

6.  Transformation and DNA size: two controlling parameters and the efficiency of the single strand intermediate.

Authors:  W R Guild; A Cato; S Lacks
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1968

7.  Origin of the residual transforming activity of denatured Bacillus subtilis DNA.

Authors:  R Rownd; D M Green; R Sternglanz; P Doty
Journal:  J Mol Biol       Date:  1968-03-14       Impact factor: 5.469

8.  Residual transforming activity of denatured Haemophilus influenzae DNA.

Authors:  M R Chevallier; G Bernardi
Journal:  J Mol Biol       Date:  1968-03-14       Impact factor: 5.469

9.  Uptake of "single-stranded" DNA in Hemophilus influenzae and its ability to transform.

Authors:  E H Postel; S H Goodgal
Journal:  J Mol Biol       Date:  1966-04       Impact factor: 5.469

10.  Transforming activity in single-stranded DNA from Bacillus subtilis.

Authors:  M D Chilton; B D Hall
Journal:  J Mol Biol       Date:  1968-06-28       Impact factor: 5.469
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  8 in total

1.  Identification of the major protein component of the pneumococcal eclipse complex.

Authors:  Donald A Morrison; Isabelle Mortier-Barrière; Laetitia Attaiech; Jean-Pierre Claverys
Journal:  J Bacteriol       Date:  2007-06-29       Impact factor: 3.490

2.  Transformation in pneumococcus: existence and properties of a complex involving donor deoxyribonucleate single strands in eclipse.

Authors:  D A Morrison
Journal:  J Bacteriol       Date:  1977-11       Impact factor: 3.490

3.  Structure of deoxyribonucleic acid on the cell surface during uptake by pneumococcus.

Authors:  D A Morrison; W R Guild
Journal:  J Bacteriol       Date:  1973-09       Impact factor: 3.490

4.  Relationship between competence for transformation of Bacillus subtilis with native and single-stranded deoxyribonucleic acid.

Authors:  M J Tevethia; C P Caudill
Journal:  J Bacteriol       Date:  1971-06       Impact factor: 3.490

5.  Genetic transformation of Streptococcus pneumoniae by DNA cloned into the single-stranded bacteriophage f1.

Authors:  F Barany; J D Boeke
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

6.  Isolation and characterization of three new classes of transformation-deficient mutants of Streptococcus pneumoniae that are defective in DNA transport and genetic recombination.

Authors:  D A Morrison; S A Lacks; W R Guild; J M Hageman
Journal:  J Bacteriol       Date:  1983-10       Impact factor: 3.490

7.  Deoxyribonucleate binding and transformation in Rhizobium jpaonicum.

Authors:  J L Raina; V V Modi
Journal:  J Bacteriol       Date:  1972-08       Impact factor: 3.490

8.  Role of the single-stranded DNA-binding protein SsbB in pneumococcal transformation: maintenance of a reservoir for genetic plasticity.

Authors:  Laetitia Attaiech; Audrey Olivier; Isabelle Mortier-Barrière; Anne-Lise Soulet; Chantal Granadel; Bernard Martin; Patrice Polard; Jean-Pierre Claverys
Journal:  PLoS Genet       Date:  2011-06-30       Impact factor: 5.917
  8 in total

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