| Literature DB >> 438654 |
P A Edwards, D Lemongello, A M Fogelman.
Abstract
A method for solubilizing HMG-CoA reductase is described that reproducibly yielded approximately 190% of the activity assayed in rat liver microsomes. Optimal solubilization occurred when microsomal membranes were frozen at a fixed concentration, thawed, homogenized in a buffer containing 50% glycerol, and incubated at 37 degrees C for 60 minutes. A rapid spectrophotometric assay of the reductase has been developed and the optimal conditions defined. Using this assay, the kinetics were determined for HMG-CoA reductase purified to a specific activity of 17,400 nmol NADPH oxidized per minute per mg protein.Entities:
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Year: 1979 PMID： 438654
Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922