Literature DB >> 6396479

A modified radiometric assay for 3-hydroxy-3-methylglutaryl coenzyme A reductase.

N H Georgopapadakou, B A Dix.   

Abstract

A radiometric assay for measuring the activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is described. The assay is based on the separation of the mevalonate product from HMG-CoA by high-voltage electrophoresis. This method is more sensitive and more specific than the NADPH-based spectrophotometric assay, and less tedious than available radiometric assays. It has been used to measure HMG-CoA reductase activity in crude extracts of Saccharomyces cerevisiae and in human skin fibroblasts.

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Year:  1984        PMID： 6396479     DOI： 10.1007/BF02534735

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  30 in total

1.  Purification and characterization of 3-hydroxy-3-methylglutaryl coenzyme A reductase from chicken liver.

Authors:  Z H Beg; J A Stonik; H B Brewer
Journal:  FEBS Lett       Date:  1977-08-01       Impact factor: 4.124

2.  Purification and properties of 3-hydroxy-3-methylglutaryl coenzyme A reductase from Pseudomonas.

Authors:  W R Bensch; V W Rodwell
Journal:  J Biol Chem       Date:  1970-08-10       Impact factor: 5.157

3.  Micro assay for 3-hydroxy-3-methylglutaryl-CoA reductase in rat liver and in L-cell fibroblasts.

Authors:  D J Shapiro; J L Nordstrom; J J Mitschelen; V W Rodwell; R T Schimke
Journal:  Biochim Biophys Acta       Date:  1974-12-29

4.  Some properties and mechanism of action of the beta-hydroxy-beta-methylglutaryl coenzyme A reductase of yeast.

Authors:  M E Kirtley; H Rudney
Journal:  Biochemistry       Date:  1967-01       Impact factor: 3.162

5.  Purification and mechanism of action of hog liver mevalonic kinase.

Authors:  E Beytia; J K Dorsey; J Marr; W W Cleland; J W Porter
Journal:  J Biol Chem       Date:  1970-10-25       Impact factor: 5.157

Review 6.  Regulation of HMG-CoA reductase.

Authors:  V W Rodwell; J L Nordstrom; J J Mitschelen
Journal:  Adv Lipid Res       Date:  1976

7.  Solubilization and purification of beta-hydroxy-beta-methylglutaryl coenzyme A reductase from rat liver.

Authors:  T Kawachi; H Rudney
Journal:  Biochemistry       Date:  1970-04-14       Impact factor: 3.162

8.  Purification and properties of rat liver 3-hydroxy-3-methylglutaryl coenzyme A reductase.

Authors:  P A Edwards; D Lemongello; A M Fogelman
Journal:  Biochim Biophys Acta       Date:  1979-07-27

9.  Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A.

Authors:  F H Hulcher; W H Oleson
Journal:  J Lipid Res       Date:  1973-11       Impact factor: 5.922

10.  3-hydroxy-3-methylglutaryl-CoA reductase: solubilization in the presence of proteolytic inhibitors, partial purification, and reversible phosphorylation-dephosphorylation.

Authors:  P J Kennelly; K G Brandt; V W Rodwell
Journal:  Biochemistry       Date:  1983-06-07       Impact factor: 3.162
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