Literature DB >> 4377219

A study of the kinetics and mechanism of rabbit muscle L-glycerol 3-phosphate dehydrogenase.

P Bentley, F M Dickinson.   

Abstract

1. The kinetics of oxidation of l-glycerol 3-phosphate by NAD(+) and of reduction of dihydroxyacetone phosphate by NADH catalysed by rabbit muscle glycerol 3-phosphate dehydrogenase were studied over the range pH6-9. 2. The enzyme was found to catalyse the oxidation of glyoxylate by NAD(+) at pH8.0 and the kinetics of this reaction were also studied. 3. The results are consistent with a compulsory mechanism of catalysis for glycerol 3-phosphate oxidation and dihydroxyacetone phosphate reduction in the intermediate regions of pH, but modifications to the basic mechanism are required to fully explain results at the extremes of the pH range, with these substrates and for glyoxylate oxidation at pH8.0.

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Year:  1974        PMID： 4377219      PMCID： PMC1168347          DOI： 10.1042/bj1430019

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  The free-energy changes for the reduction of diphosphopyridine nucleotide and the dehydrogenation of L-malate and L-glycerol 1-phosphate.

Authors:  K BURTON; T H WILSON
Journal:  Biochem J       Date:  1953-04       Impact factor: 3.857

2.  Studies on the mechanism of enzyme-catalyzed oxidation reduction reactions. IV. A proposed mechanism for the over-all reaction catalyzed by liver alcohol dehydrogenase.

Authors:  H R MAHLER; R H BAKER; V J SHINER
Journal:  Biochemistry       Date:  1962-01       Impact factor: 3.162

3.  Kinetic studies on the mechanism of cytoplasmic L-alpha-glycerophosphate dehydrogenase of rabbit skeletal muscle.

Authors:  W J Black
Journal:  Can J Biochem       Date:  1966-10

4.  Purification and properties of rabbit muscle L-glycerol 3-phosphate dehydrogenase.

Authors:  P Bentley; F M Dickinson; I G Jones
Journal:  Biochem J       Date:  1973-12       Impact factor: 3.857

5.  Structural studies on rabbit muscle glycerol 3-phosphate dehydrogenase and a comparison of chemical and physical determinations of its molecular weight.

Authors:  T P Fondy; C R Ross; S J Sollohub
Journal:  J Biol Chem       Date:  1969-03-25       Impact factor: 5.157

6.  Isolation and characterization of cytoplasmic L-glycerol-3-phosphate dehydrogenase from rabbit-renal-adipose tissue and its comparison with the skeletal-muscle enzyme.

Authors:  D L Warkentin; T P Fondy
Journal:  Eur J Biochem       Date:  1973-07-02

7.  Protein fluorescence of nicotinamide nucleotide-dependent dehydrogenases.

Authors:  J J Holbrook; D W Yates; S J Reynolds; R W Evans; C Greenwood; M G Gore
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

8.  A study of the kinetics and mechanism of yeast alcohol dehydrogenase with a variety of substrates.

Authors:  F M Dickinson; G P Monger
Journal:  Biochem J       Date:  1973-02       Impact factor: 3.857

9.  A study of the coenzyme-binding characteristics of rabbit muscle L-glycerol 3-phosphate dehydrogenase.

Authors:  P Bentley; F M Dickinson
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

10.  Dihydroxyacetone phosphate. Its structure and reactivity with -glycerophosphate dehydrogenase, aldolase and triose phosphate isomerase and some possible metabolic implications.

Authors:  S J Reynolds; D W Yates; C I Pogson
Journal:  Biochem J       Date:  1971-04       Impact factor: 3.857
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  8 in total

1.  A study of the pH- and temperature-dependence of the reactions of yeast alcohol dehydrogenase with ethanol, acetaldehyde and butyraldehyde as substrates.

Authors:  C J Dickenson; F M Dickinson
Journal:  Biochem J       Date:  1975-05       Impact factor: 3.857

2.  Substrate channeling in glycolysis: a phantom phenomenon.

Authors:  X M Wu; H Gutfreund; S Lakatos; P B Chock
Journal:  Proc Natl Acad Sci U S A       Date:  1991-01-15       Impact factor: 11.205

3.  A study of the coenzyme-binding characteristics of rabbit muscle L-glycerol 3-phosphate dehydrogenase.

Authors:  P Bentley; F M Dickinson
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857

4.  A substrate in pieces: allosteric activation of glycerol 3-phosphate dehydrogenase (NAD+) by phosphite dianion.

Authors:  Wing-Yin Tsang; Tina L Amyes; John P Richard
Journal:  Biochemistry       Date:  2008-04-01       Impact factor: 3.162

5.  Enzyme architecture: optimization of transition state stabilization from a cation-phosphodianion pair.

Authors:  Archie C Reyes; Astrid P Koudelka; Tina L Amyes; John P Richard
Journal:  J Am Chem Soc       Date:  2015-04-21       Impact factor: 15.419

6.  Primary Deuterium Kinetic Isotope Effects: A Probe for the Origin of the Rate Acceleration for Hydride Transfer Catalyzed by Glycerol-3-Phosphate Dehydrogenase.

Authors:  Archie C Reyes; Tina L Amyes; John P Richard
Journal:  Biochemistry       Date:  2018-07-10       Impact factor: 3.162

7.  Modeling the Role of a Flexible Loop and Active Site Side Chains in Hydride Transfer Catalyzed by Glycerol-3-phosphate Dehydrogenase.

Authors:  Anil R Mhashal; Adrian Romero-Rivera; Lisa S Mydy; Judith R Cristobal; Andrew M Gulick; John P Richard; Shina C L Kamerlin
Journal:  ACS Catal       Date:  2020-09-03       Impact factor: 13.084

8.  Hydride Transfer Catalyzed by Glycerol Phosphate Dehydrogenase: Recruitment of an Acidic Amino Acid Side Chain to Rescue a Damaged Enzyme.

Authors:  Rui He; Judith R Cristobal; Naiji Jabin Gong; John P Richard
Journal:  Biochemistry       Date:  2020-12-11       Impact factor: 3.162
  8 in total

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