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Literature DB >> 4372416

In vitro radioisotopic labeling of proteins associated with purified polyoma virions.

Abstract

An in vitro procedure using [(14)C]formaldehyde and sodium borohydride was used to label the proteins of purified polyoma virions. This method, which labeled the three capsid proteins and the four internal histones of polyoma virus, did not alter the biological and biophysical characteristics of the virus. In addition to the seven viral proteins, five additional radioactive peaks were resolved on polyacrylamide gels. Four of these peaks could be attributed to growing the virus in the presence of serum. The fifth peak appears to be a nonhistone host-contributed protein made before infection.

Entities: Chemical Disease

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Year: 1974 PMID： 4372416 PMCID： PMC355699

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

18 in total

1. Transient inhibition of polyoma virus synthesis by Sendai virus (parainfluenza I). I. Demonstration and nature of the inhibition by inactivated virus.

Authors: G L Smith; R A Consigli
Journal: J Virol Date: 1972-12 Impact factor: 5.103

1. Radioactive labelling of ribosomal proteins with reductive alkylation and its use in studying ribosome-cytosol interactions.

Authors: C J Kelly; T C Johnson
Journal: Biochem J Date: 1976-12-15 Impact factor: 3.857

8. Characterization of a DNA-protein complex and capsomere subunits derived from polyoma virus by treatment with ethyleneglycol-bis-N,N'-tetraacetic acid and dithiothreitol.

Authors: J N Brady; V D Winston; R A Consigli
Journal: J Virol Date: 1978-07 Impact factor: 5.103

9. Isolation of a transcriptive complex from Newcastle disease virions.

Authors: R J Colonno; H O Stone
Journal: J Virol Date: 1976-09 Impact factor: 5.103

10. Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions.

Authors: J N Brady; V D Winston; R A Consigli
Journal: J Virol Date: 1977-09 Impact factor: 5.103

In vitro radioisotopic labeling of proteins associated with purified polyoma virions.

1. Transient inhibition of polyoma virus synthesis by Sendai virus (parainfluenza I). I. Demonstration and nature of the inhibition by inactivated virus.

2. Specific labelling of virus proteins by quantitative reaction of cysteine residues with 35 S-sulphite.

3. Cytologic studies in polyoma virus-infected mouse embryo cells.

4. Multiplication of polyoma virus. II. Source of constituents for viral deoxyribonucleic acid and protein synthesis.

5. A specific labeling procedure for proteins on the outer surface of membranes.

6. The polypeptides of influenza virus. V. Localization of polypeptides in the virion by iodination techniques.

7. The arrangement of proteins in the human erythrocyte membrane.

8. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

9. An enzymic method for the trace iodination of immunoglobulins and other proteins.

10. The enzymatic iodination of the red cell membrane.

1. Radioactive labelling of ribosomal proteins with reductive alkylation and its use in studying ribosome-cytosol interactions.

2. Chromatographic separation of the polyoma virus proteins and renaturation of the isolated VP1 major capsid protein.

3. Origin of the polyoma virus-associated endonuclease.

4. Immunological reactivity of antisera to sodium dodecyl sulfate-derived polypeptides of polyoma virions.

5. Dynamic association of gammaherpesvirus DNA with core histone during de novo lytic infection of primary cells.

6. Radioisotopic labeling of human papovavirus (BK) by iodination and reductive alkylation.

7. Early events in polyoma virus infection: attachment, penetration, and nuclear entry.

8. Characterization of a DNA-protein complex and capsomere subunits derived from polyoma virus by treatment with ethyleneglycol-bis-N,N'-tetraacetic acid and dithiothreitol.

9. Isolation of a transcriptive complex from Newcastle disease virions.

10. Dissociation of polyoma virus by the chelation of calcium ions found associated with purified virions.