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Literature DB >> 4354740

Affinity chromatography of lactate dehydrogenase on immobilized nucleotides.

Abstract

The interaction of two isoenzymes of lactate dehydrogenase from pig heart muscle (H(4)) and rabbit skeletal muscle (M(4)), with immobilized nucleotides was examined: the effects of pH and temperature on the binding of lactate dehydrogenase were studied with immobilized NAD(+) matrices. The influence of substrate, product and sulphite on the binding of heart muscle lactate dehydrogenase to immobilized NAD(+) was investigated. The interaction of both lactate dehydrogenase isoenzymes with immobilized pyridine and adenine nucleotides and their derivatives were measured. The effects of these parameters on the interaction of lactate dehydrogenase with immobilized nucleotides were correlated with the known kinetic and molecular properties of the enzymes in free solution.

Entities: Chemical Species

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Year: 1973 PMID： 4354740 PMCID： PMC1177729 DOI： 10.1042/bj1330515

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

21 in total

1. Affinity chromatography of enzymes on insolubilized cofactors.

Authors: C R. Lowe; P D.G. Dean
Journal: FEBS Lett Date: 1971-05-20 Impact factor: 4.124

2. Lactic dehydrogenase. IV. The influence of pH on the kinetics of the reaction.

Authors: A D WINER; G W SCHWERT
Journal: J Biol Chem Date: 1958-04 Impact factor: 5.157

3. Lactic dehydrogenase. II. Variation of kinetic and equilibrium constants with temperature.

Authors: M T HAKALA; A J GLAID; G W SCHWERT
Journal: J Biol Chem Date: 1956-07 Impact factor: 5.157

4. Some parameters relevant to affinity chromatography on immobilized nucleotides.

Authors: C R Lowe; M J Harvey; D B Craven; P D Dean
Journal: Biochem J Date: 1973-07 Impact factor: 3.857

5. The resolution of active and inactive xanthine oxidase by affinity chromatography.

Authors: D Edmondson; V Massey; G Palmer; L M Beacham; G B Elion
Journal: J Biol Chem Date: 1972-03-10 Impact factor: 5.157

6. Preparation of a NAD(H)-polymer matrix showing coenzyme function of the bound pyridine nucleotide.

Authors: P O Larsson; K Mosbach
Journal: Biotechnol Bioeng Date: 1971-05 Impact factor: 4.530

7. Separation of regulatory and catalytic subunits of the cyclic 3',5'-adenosine monophosphate-dependent protein kinase(s) of rabbit skeletal muscle.

Authors: E M Reimann; C O Brostrom; J D Corbin; C A King; E G Krebs
Journal: Biochem Biophys Res Commun Date: 1971-01-22 Impact factor: 3.575

Review 8. Affinity chromatography.

Authors: P Cuatrecasas
Journal: Annu Rev Biochem Date: 1971 Impact factor: 23.643

9. The importance of SH-groups for enzymic activity. V. The coenzyme-binding capacity of pig heart lactate dehydrogenase, isozyme I, after inhibition by various maleinimides.

Authors: J J Holbrook
Journal: Biochem Z Date: 1966-03-28