Literature DB >> 4346886

Effect of environmental pH on rescue of Simian virus 40.

G Calothy, C M Croce, V Defendi, H Koprowski, H Eagle.   

Abstract

Rescue of SV40 virus after Sendai virus-mediated fusion of transformed mouse or hamster cell lines with permissive monkey cells was strikingly dependent on pH in the range 6.4-8.8, with a maximum at pH 8.4. The titer of virus recovered at pH 8.4 was 2 logs higher than that at pH 7.6, and 4 logs higher than that at pH 6.4. The pH-sensitive step was neither the number of heterokaryocytes formed, which was essentially the same at pH 7.6 and 8.4, nor the degree of SV40 replication in the monkey cells, which was also unaffected by pH variation in the range 7.2-8.4.

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Year:  1973        PMID： 4346886      PMCID： PMC433260          DOI： 10.1073/pnas.70.2.366

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  10 in total

1.  Amino acid metabolism in mammalian cell cultures.

Authors:  H EAGLE
Journal:  Science       Date:  1959-08-21       Impact factor: 47.728

2.  INFECTION OF HUMAN AND SIMIAN TISSUE CULTURES WITH ROUS SARCOMA VIRUS.

Authors:  F C JENSEN; A J GIRARDI; R V GILDEN; H KOPROWSKI
Journal:  Proc Natl Acad Sci U S A       Date:  1964-07       Impact factor: 11.205

3.  Effect of environmental pH on the efficiency of cellular hybridization.

Authors:  C M Croce; H Koprowski; H Eagle
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205

4.  Genetic analysis of simian virus 40. I. Description of microtitration and replica-plating techniques for virus.

Authors:  J A Robb; R G Martin
Journal:  Virology       Date:  1970-08       Impact factor: 3.616

5.  Production of SV40 virus in heterokaryons of transformed and susceptible cells.

Authors:  J F Watkins; R Dulbecco
Journal:  Proc Natl Acad Sci U S A       Date:  1967-10       Impact factor: 11.205

6.  Activation of production of infectious tumor virus SV40 in heterokaryon cultures.

Authors:  H Koprowski; F C Jensen; Z Steplewski
Journal:  Proc Natl Acad Sci U S A       Date:  1967-07       Impact factor: 11.205

7.  Buffer combinations for mammalian cell culture.

Authors:  H Eagle
Journal:  Science       Date:  1971-10-29       Impact factor: 47.728

8.  Hydrogen ion buffers for biological research.

Authors:  N E Good; G D Winget; W Winter; T N Connolly; S Izawa; R M Singh
Journal:  Biochemistry       Date:  1966-02       Impact factor: 3.162

9.  Virogenic properties of bromodeoxyuridine-sensitive and bromodeoxyuridine-resistant simian virus 40-transformed mouse kidney cells.

Authors:  D R Dubbs; S Kit; R A De Torres; M Anken
Journal:  J Virol       Date:  1967-10       Impact factor: 5.103

10.  HYBRID CELLS DERIVED FROM MOUSE AND MAN: ARTIFICIAL HETEROKARYONS OF MAMMALIAN CELLS FROM DIFFERENT SPECIES.

Authors:  H HARRIS; J F WATKINS
Journal:  Nature       Date:  1965-02-13       Impact factor: 49.962
  10 in total
  2 in total

1.  Effect of pH on plating efficiency, serum reguirement, and incorporation of radioactive precursors into human cells.

Authors:  C Ceccarini
Journal:  In Vitro       Date:  1975 Mar-Apr

2.  Assignment of the T-antigen gene of simian virus 40 to human chromosome C-7.

Authors:  C M Croce; A J Girardi; H Koprowski
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205
  2 in total

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