Literature DB >> 4344026

Immunization of mice against encephalomyocarditis virus. I. Purification, concentration, and inactivation of encephalomyocarditis virus.

W J Bogaerts.   

Abstract

Methods are described for the concentration, purification, and inactivation of encephalomyocarditis virus grown in L-cell suspension cultures. Virus concentrates were obtained by the precipitation of crude virus lysates with polyethylene glycol and were purified by Genetron extraction. After column chromatography approximately 70% recovery of infectivity and hemagglutination (HA) activity was found. Exposure of the purified virus suspensions to ultraviolet light resulted in reduction of the infectivity by a factor of 10(9) with little or no loss of HA activity. The preparations are being evaluated as vaccines.

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Year:  1972        PMID： 4344026      PMCID： PMC422566          DOI： 10.1128/iai.6.4.508-512.1972

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  16 in total

1.  Studies on poliomyelitis virus. III. The use of ultra-violet light as an additional means of inactivation of formalinized vaccine.

Authors:  J W HAMPTON; A POLSON
Journal:  J Hyg (Lond)       Date:  1958-06

2.  Experimental respiratory infection with type 4 adenovirus vaccine in volunteers: clinical and immunological responses.

Authors:  T J Smith; E L Buescher; F H Top; W A Altemeier; J M McCown
Journal:  J Infect Dis       Date:  1970-10       Impact factor: 5.226

3.  [Studies on precipitation, ion exchange chromatography and electrophoretic mobility of tissue-culture viruses in the presence of water soluble linear polycondensation and polymerisation products].

Authors:  W Matthaeus
Journal:  Zentralbl Bakteriol Orig       Date:  1969-05

4.  The immunologic response of the mouse to aerosols of an attenuated encephalomyocarditis virus strain.

Authors:  C M Prato; T G Akers
Journal:  J Immunol       Date:  1969-07       Impact factor: 5.422

5.  Poliovirus antibody response in serum and nasal secretions following intranasal inoculation with inactivated poliovaccine.

Authors:  P L Ogra; D T Karzon
Journal:  J Immunol       Date:  1969-01       Impact factor: 5.422

6.  Large-scale preparations of viruses by steric chromatography on columns of controlled pore glass. Phi-X174, M13, M12, Q-beta and T4 bacteriophages.

Authors:  H H Gschwender; W Haller; P H Hofschneider
Journal:  Biochim Biophys Acta       Date:  1969-10-22

7.  [Concentration of virus by polyethylene-glycol].

Authors:  G Calothy; M Digeon; M Raynaud
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1967-11-27

8.  Neuraminidase- and hemagglutinin-inhibiting antibodies in serum and nasal secretions of volunteers immunized with attenuated and inactivated influenza B-Eng-13-65 virus vaccines.

Authors:  J C Downie
Journal:  J Immunol       Date:  1970-09       Impact factor: 5.422

9.  Antibody responses to and efficacy of an inactivated spray vaccine.

Authors:  A S Beare; D Hobson; S E Reed; D A Tyrrell
Journal:  Bull World Health Organ       Date:  1969       Impact factor: 9.408

10.  Testing the safety and effectiveness of oral administration of a live influenza vaccine.

Authors:  G I Aleksandrova; A A Smorodintsev; N M Beljaeva; B J Vasil'ev; R A Geft; V G Panteleev; M A Sejfer; A A Selivanov
Journal:  Bull World Health Organ       Date:  1970       Impact factor: 9.408
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  4 in total

1.  Inactivation of encephalomyocarditis virus in aerosols: fate of virus protein and ribonucleic acid.

Authors:  J C de Jong; M Harmsen; T Trouwborst; K C Winkler
Journal:  Appl Microbiol       Date:  1974-01

2.  Immunization of mice against encephalomyocarditis virus. II. Intraperitoneal and respiratory immunization with ultraviolet-inactivated vaccine: effect of Bordetella pertussis extract on the immune response.

Authors:  W J Bogaerts
Journal:  Infect Immun       Date:  1972-10       Impact factor: 3.441

3.  Immunization of mice with live attenuated encephalomyocarditis virus: local immunity and survival.

Authors:  W J Bogaerts
Journal:  Infect Immun       Date:  1973-10       Impact factor: 3.441

4.  A rapid Orthopoxvirus purification protocol suitable for high-containment laboratories.

Authors:  Laura Hughes; Kimberly Wilkins; Cynthia S Goldsmith; Scott Smith; Paul Hudson; Nishi Patel; Kevin Karem; Inger Damon; Yu Li; Victoria A Olson; P S Satheshkumar
Journal:  J Virol Methods       Date:  2017-01-26       Impact factor: 2.623
  4 in total

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