Failure to Detect Cyclic 3', 5'-Adenosine Monophosphate in Healthy and Crown Gall Tumorous Tissues of Vicia faba.

Attempts were made to provide proof for the occurrence of cyclic 3',5'-adenosine monophosphate in healthy and crown gall tissues of Vicia faba. Although our purified extracts gave positive readings in the Gilman binding assay for cyclic AMP, they were not digested by a specific cyclic 3',5'-adenosine monophosphate phosphodiesterase from beef heart. The extracts were digested, however, by a partially purified cyclic nucleotide phosphodiesterase from carrot tissue, which attacks both cyclic 2',3'- and 3',5'-nucleotides. The data indicate that the substances detected in the V. faba extracts are perhaps cyclic 2',3'-nucleotides, a possible RNA degradation product.