Literature DB >> 4318774

Identification of proinsulin and C-peptide in human serum by a specific immunoassay.

F Melani, A H Rubenstein, P E Oyer, D F Steiner.   

Abstract

An immunoassay for the measurement of human proinsulin and C-peptide is described. By a combination of this system and an insulin immunoassay, proinsulin, insulin, and C-peptide were identified in acid-ethanol extracts of serum after gel filtration. In keeping with the results of biosynthetic studies, insulin and C-peptide were found to be present in equimolar amounts. The absolute values of serum proinsulin determined independently in the two assays corresponded closely.

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Year:  1970        PMID： 4318774      PMCID： PMC283181          DOI： 10.1073/pnas.67.1.148

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

Review 1.  The pancreatic beta cell. Structure and function.

Authors:  P E Lacy
Journal:  N Engl J Med       Date:  1967-01-26       Impact factor: 91.245

2.  Isolation of a proinsulin connecting peptide fragment (C-peptide) from bovine and human pancreas.

Authors:  J L Clark; S Cho; A H Rubenstein; D F Steiner
Journal:  Biochem Biophys Res Commun       Date:  1969-05-22       Impact factor: 3.575

3.  Concepts relating to the structure of protein hormones.

Authors:  R E Canfield
Journal:  Ann N Y Acad Sci       Date:  1968-02-05       Impact factor: 5.691

4.  Preparation and characterization of poly-DL-alanyl rabbit gamma-globulin.

Authors:  S Fuchs; M Sela
Journal:  J Biol Chem       Date:  1965-09       Impact factor: 5.157

5.  Human serum proinsulin.

Authors:  F Melani; A H Rubenstein; D F Steiner
Journal:  J Clin Invest       Date:  1970-03       Impact factor: 14.808

6.  ANTIBODIES TO BRADYKININ AND ANGIOTENSIN: A USE OF CARBODIIMIDES IN IMMUNOLOGY.

Authors:  T L GOODFRIEND; L LEVINE; G D FASMAN
Journal:  Science       Date:  1964-06-12       Impact factor: 47.728

7.  Porcine proinsulin: characterization and amino acid sequence.

Authors:  R E Chance; R M Ellis; W W Bromer
Journal:  Science       Date:  1968-07-12       Impact factor: 47.728

8.  The gastrointestinal stimulus to insulin release. I. Secretin.

Authors:  D J Chisholm; J D Young; L Lazarus
Journal:  J Clin Invest       Date:  1969-08       Impact factor: 14.808

Review 9.  Proinsulin and the biosynthesis of insulin.

Authors:  D F Steiner; J L Clark; C Nolan; A H Rubenstein; E Margoliash; B Aten; P E Oyer
Journal:  Recent Prog Horm Res       Date:  1969

10.  The biological and immunological properties of pork and beef insulin, proinsulin, and connecting peptides.

Authors:  A E Kitabchi
Journal:  J Clin Invest       Date:  1970-05       Impact factor: 14.808
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  43 in total

1.  Human C-peptide in normal and diabetic subjects.

Authors:  L G Heding; S M Rasmussen
Journal:  Diabetologia       Date:  1975-06       Impact factor: 10.122

2.  Proinsulin, insulin, and C-peptide concentrations in human portal and peripheral blood.

Authors:  D L Horwitz; J I Starr; M E Mako; W G Blackard; A H Rubenstein
Journal:  J Clin Invest       Date:  1975-06       Impact factor: 14.808

3.  B-cell function and blood glucose control in insulin dependent diabetics within the first month of insulin treatment.

Authors:  O K Faber; C Binder
Journal:  Diabetologia       Date:  1977-05       Impact factor: 10.122

4.  Indirect two-site immunoradiometric assay of rat and mouse proinsulin.

Authors:  D K Yue; O M Gibby; S D Luzio; N Yanaihara; C N Hales
Journal:  Diabetologia       Date:  1979-10       Impact factor: 10.122

5.  Specific and direct radioimmunoassay for human proinsulin in serum.

Authors:  L G Heding
Journal:  Diabetologia       Date:  1977-09       Impact factor: 10.122

6.  Donald F. Steiner MD, 1930-2014: discoverer of proinsulin.

Authors:  Louis H Philipson; Graeme Bell; Kenneth S Polonsky
Journal:  Proc Natl Acad Sci U S A       Date:  2015-01-05       Impact factor: 11.205

7.  Insulin, C-peptide and glucagon levels during OGTT in hepatic cirrhosis and in patients with prehepatic block.

Authors:  L Gerö; L Korányi; F Szalay; B Büki; G Tamás
Journal:  Acta Diabetol Lat       Date:  1982 Jan-Mar

8.  Human C-peptide. Part II: Clinical studies.

Authors:  W Beischer; E Heinze; L Keller; S Raptis; W Kerner; E F Pfeiffer
Journal:  Klin Wochenschr       Date:  1976-08-01

9.  Human C-peptide immunoreactivity (CPR) in blood and urine - evaluation of a radioimmunoassay method and its clinical applications.

Authors:  T Kuzuya; T Saito; S Yoshida; A Matsuda
Journal:  Diabetologia       Date:  1976-10       Impact factor: 10.122

10.  Kinetics of human connecting peptide in normal and diabetic subjects.

Authors:  O K Faber; C Hagen; C Binder; J Markussen; V K Naithani; P M Blix; H Kuzuya; D L Horwitz; A H Rubenstein; N Rossing
Journal:  J Clin Invest       Date:  1978-07       Impact factor: 14.808
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