Literature DB >> 4315158

Characterization of bluetongue virus ribonucleic acid.

D W Verwoerd, H Louw, R A Oellermann.   

Abstract

An improved purification procedure yielded bluetongue virus free from any single-stranded ribonucleic acid (RNA) component. Double-stranded RNA obtained from purified virus or isolated from infected cells was fractionated into 5 components by means of sucrose gradient sedimentation analysis, and into 10 components by electrophoresis on polyacrylamide gels. The size of these components vary from 0.5 x 10(6) to 2.8 x 10(6) daltons, with a total molecular weight estimate of about 1.5 x 10(7) for the viral nucleic acid. The denaturation of the genome and separation of the resulting fragments are also discussed.

Entities:  

Mesh：

Substances：

Year:  1970        PMID： 4315158      PMCID： PMC375961     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  13 in total

1.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

2.  Sedimentation characteristics of rapidly labelled RNA from HeLa cells.

Authors:  K SCHERRER; J E DARNELL
Journal:  Biochem Biophys Res Commun       Date:  1962-06-04       Impact factor: 3.575

3.  Purification and characterization of bluetongue virus.

Authors:  D W Verwoerd
Journal:  Virology       Date:  1969-06       Impact factor: 3.616

4.  Plaque formation by bluetongue virus.

Authors:  P G Howell; D W Verwoerd; R A Oellermann
Journal:  Onderstepoort J Vet Res       Date:  1967-12       Impact factor: 1.792

5.  Regulation of transcription of the Reovirus genome.

Authors:  Y Watanabe; S Millward; A F Graham
Journal:  J Mol Biol       Date:  1968-08-28       Impact factor: 5.469

6.  Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.

Authors:  A J Shatkin; J D Sipe; P Loh
Journal:  J Virol       Date:  1968-10       Impact factor: 5.103

7.  Single-stranded, adenine-rich RNA from purified reoviruses.

Authors:  A J Shatkin; J D Sipe
Journal:  Proc Natl Acad Sci U S A       Date:  1968-01       Impact factor: 11.205

8.  Tables for estimating sedimentation through linear concentration gradients of sucrose solution.

Authors:  C R McEwen
Journal:  Anal Biochem       Date:  1967-07       Impact factor: 3.365

9.  Studies on reovirus RNA. I. Characterization of reovirus genome RNA.

Authors:  A R Bellamy; L Shapiro; J T August; W K Joklik
Journal:  J Mol Biol       Date:  1967-10-14       Impact factor: 5.469

10.  Structural units of reovirus ribonucleic acid and their possible functional significance.

Authors:  Y Watanabe; A F Graham
Journal:  J Virol       Date:  1967-08       Impact factor: 5.103
View more
  44 in total

1.  Nucleotide sequence of the genome segment encoding nonstructural protein NS1 of bluetongue virus serotype 20 from Australia.

Authors:  J A Cowley
Journal:  Virus Genes       Date:  1992-11       Impact factor: 2.332

2.  Electrophoretic separation of double-stranded RNA genome segments from Warrego and Mitchell River viruses.

Authors:  B M Gorman; P J Walker; J Taylor
Journal:  Arch Virol       Date:  1977       Impact factor: 2.574

3.  Continuity of the dsRNA genome of bluetongue virus.

Authors:  N M Foster; M A Alders; T E Walton
Journal:  Curr Microbiol       Date:  1978       Impact factor: 2.188

4.  Expression of largest RNA segment and synthesis of VP1 protein of bluetongue virus in insect cells by recombinant baculovirus: association of VP1 protein with RNA polymerase activity.

Authors:  T Urakawa; D G Ritter; P Roy
Journal:  Nucleic Acids Res       Date:  1989-09-25       Impact factor: 16.971

5.  Development of reverse genetics systems for bluetongue virus: recovery of infectious virus from synthetic RNA transcripts.

Authors:  Mark Boyce; Cristina C P Celma; Polly Roy
Journal:  J Virol       Date:  2008-06-18       Impact factor: 5.103

6.  Full genome characterisation of bluetongue virus serotype 6 from the Netherlands 2008 and comparison to other field and vaccine strains.

Authors:  Sushila Maan; Narender S Maan; Piet A van Rijn; René G P van Gennip; Anna Sanders; Isabel M Wright; Carrie Batten; Bernd Hoffmann; Michael Eschbaumer; Chris A L Oura; Abraham C Potgieter; Kyriaki Nomikou; Peter P C Mertens
Journal:  PLoS One       Date:  2010-04-23       Impact factor: 3.240

7.  RT-PCR assays for seven serotypes of epizootic haemorrhagic disease virus & their use to type strains from the Mediterranean region and North America.

Authors:  Narender S Maan; Sushila Maan; Kyriaki Nomikou; Donna J Johnson; Mehdi El Harrak; Hafsa Madani; Hagai Yadin; Serife Incoglu; Kadir Yesilbag; Andrew B Allison; David E Stallknecht; Carrie Batten; Simon J Anthony; Peter P C Mertens
Journal:  PLoS One       Date:  2010-09-17       Impact factor: 3.240

8.  Genetic modification of Bluetongue virus by uptake of "synthetic" genome segments.

Authors:  René G P van Gennip; Daniel Veldman; Sandra G P van de Water; Piet A van Rijn
Journal:  Virol J       Date:  2010-10-07       Impact factor: 4.099

9.  Isolation and identification of a variant of bluetongue virus serotype 11 from a ram in a bluetongue outbreak in western Texas.

Authors:  S I Chung; P Billingsley; C W Livingston; E W Collisson
Journal:  J Clin Microbiol       Date:  1991-07       Impact factor: 5.948

10.  Establishment of a bluetongue virus infection model in mice that are deficient in the alpha/beta interferon receptor.

Authors:  Eva Calvo-Pinilla; Teresa Rodríguez-Calvo; Juan Anguita; Noemí Sevilla; Javier Ortego
Journal:  PLoS One       Date:  2009-04-09       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.