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Literature DB >> 1275901

Artifacts in the centrifugation of ribosomal and heterogenous ribonucleic acid in "99%-dimethyl sulphoxide" gradients.

Abstract

Increasing the total quantity of RNA located on 99%-dimethyl sulphoxide gradients profoundly altered the sedimentation pattern of heterogenous RNA and (to a smaller extent) that of rRNA. Aggregation of small quantities of rRNA with fast-sedimenting heterogenous RNA was also found to occur in these gradients.

Entities: Chemical

Mesh：

Substances：

Year: 1976 PMID： 1275901 PMCID： PMC1172603 DOI： 10.1042/bj1530509

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

13 in total

1. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID.

Authors: K S KIRBY
Journal: Biochem J Date: 1965-07 Impact factor: 3.857

2. Size distribution and cell-free translation of globin-coding HnRNA from avian erythroblasts.

Authors: W Knöchel; H Tiedemann
Journal: Biochim Biophys Acta Date: 1975-02-10

3. Failure of dimethylsulfoxide to remove all secondary structure from ribosomal RNA.

Authors: S R De Kloet; V S Mayo; B A Andrean
Journal: Arch Biochem Biophys Date: 1974-05 Impact factor: 4.013

4. Molecular weight determination of Sendai and Newcastle disease virus RNA.

Authors: D Kolakofsky; E Boy de la Tour; H Delius
Journal: J Virol Date: 1974-02 Impact factor: 5.103

5. Demonstration of globin messenger sequences in giant nuclear precursors of messenger RNA of avian erythroblasts.

Authors: T Imaizumi; H Diggelmann; K Scherrer
Journal: Proc Natl Acad Sci U S A Date: 1973-04 Impact factor: 11.205

6. Cell-free translation of the globin message within polydisperse high-molecular-weight ribonucleic acid of avian erythrocytes.

Authors: A Ruiz-Carrilo; M Beato; G Schutz; P Feigelson; V G Allfrey
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

1. Intracellular distribution and sedimentation properties of virus-specific RNA in two clones of BHK cells transformed by polyoma virus.

Authors: I H Maxwell
Journal: J Virol Date: 1976-05 Impact factor: 5.103

1 in total

Artifacts in the centrifugation of ribosomal and heterogenous ribonucleic acid in "99%-dimethyl sulphoxide" gradients.

1. ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID.

2. Size distribution and cell-free translation of globin-coding HnRNA from avian erythroblasts.

3. Failure of dimethylsulfoxide to remove all secondary structure from ribosomal RNA.

4. Molecular weight determination of Sendai and Newcastle disease virus RNA.

5. Demonstration of globin messenger sequences in giant nuclear precursors of messenger RNA of avian erythroblasts.

6. Cell-free translation of the globin message within polydisperse high-molecular-weight ribonucleic acid of avian erythrocytes.

7. Preparation, molecular weight, base composition, and secondary structure of giant nuclear ribonucleic acid.

8. Replication of Sindbis virus. I. Relative size and genetic content of 26 s and 49 s RNA.

9. Denaturation of RNA with dimethyl sulfoxide.

10. Isolation of deoxyribonucleic acid from mammalian tissues.

1. Intracellular distribution and sedimentation properties of virus-specific RNA in two clones of BHK cells transformed by polyoma virus.