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Literature DB >> 4289358

Enhancement of rhinovirus plaque formation in human heteroploid cell cultures by magnesium and calcium.

Abstract

Fiala, Milan (University of Washington, Seattle), and George E. Kenny. Enhancement of rhinovirus plaque formation in human heteroploid cell cultures by magnesium and calcium. J. Bacteriol. 92:1710-1715. 1966.-A reproducible macroplaque assay for six M and three H strains of rhinoviruses has been developed in several human heteroploid cell lines. Plaques were produced only with suitable solidifying agents: purified agar (Ionagar, Agarose) or methylcellulose. Plaque development was greatly enhanced by increasing Mg(+2) to 30 to 40 mm. Diethylaminoethyl (DEAE) dextran also increased plaque sizes, and the effects of Mg(+2) and DEAE dextran were additive. In addition, Ca(+2) substituted for Mg(+2). The suitability of human heteroploid cell lines for rhinovirus plaque assay varied greatly, ranging from insensitivity through partial to complete sensitivity. This assay was six to seven times more sensitive than an end point tube assay. These results indicate that potentiation of plaque formation by Mg(+2) known for some enteroviruses can also be extended to the rhinovirus group of picornaviruses.

Entities: Chemical Species

Mesh：

Substances：
Dextrans
Magnesium
Calcium

Year: 1966 PMID： 4289358 PMCID： PMC316252 DOI： 10.1128/jb.92.6.1710-1715.1966

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

1. A DESCRIPTION OF TWO NEWLY-RECOGNIZED RHINOVIRUSES OF HUMAN ORIGIN.

Authors: P A WEBB; K M JOHNSON; M A MUFSON
Journal: Proc Soc Exp Biol Med Date: 1964-07

2. THE BASIS FOR THE SIZE DIFFERENCES IN PLAQUES PRODUCED BY VARIANTS OF ENCEPHALOMYOCARDITIS (EMC) VIRUS.

Authors: H LIEBHABER; K K TAKEMOTO
Journal: Virology Date: 1963-08 Impact factor: 3.616

3. Magnesium chloride enhancement of cell susceptibility to poliovirus.

Authors: C WALLIS; J L MELNICK
Journal: Virology Date: 1962-02 Impact factor: 3.616

4. Serotypes of viruses (rhinoviruses) isolated from common colds.

Authors: D TAYLOR-ROBINSON; D A TYRRELL
Journal: Lancet Date: 1962-03-03 Impact factor: 79.321

5. Serological studies on some viruses isolated from common colds (rhinoviruses).

Authors: D TAYLOR-ROBINSON; D A TYRRELL
Journal: Br J Exp Pathol Date: 1962-06

6. An agar polysaccharide and d marker of poliovirus.

Authors: V I AGOL; M Ia CHUMAKOVA
Journal: Virology Date: 1962-05 Impact factor: 3.616

7. A plaque method for assaying some viruses isolated from common colds.

Authors: R PARSONS; D A TYRRELL
Journal: Nature Date: 1961-02-25 Impact factor: 49.962

8. Improved method for staining cell monolayers for virus plaque counts.

Authors: J J HOLLAND; L C McLAREN
Journal: J Bacteriol Date: 1959-10 Impact factor: 3.490

9. A Case of Temporal Arteritis (Horton-Magath Syndrome).

Authors: P H Sprague; W C Mackenzie
Journal: Can Med Assoc J Date: 1940-12 Impact factor: 8.262

10. Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin.

Authors: C Wallis; F Morales; J Powell; J L Melnick
Journal: J Bacteriol Date: 1966-05 Impact factor: 3.490

29 in total

1. Temperature-dependent innate defense against the common cold virus limits viral replication at warm temperature in mouse airway cells.

Authors: Ellen F Foxman; James A Storer; Megan E Fitzgerald; Bethany R Wasik; Lin Hou; Hongyu Zhao; Paul E Turner; Anna Marie Pyle; Akiko Iwasaki
Journal: Proc Natl Acad Sci U S A Date: 2015-01-05 Impact factor: 11.205

10. Demonstration of dual rhinovirus infection in humans by isolation of different serotypes in human heteroploid (HeLa) and human diploid fibroblast cell cultures.

Authors: M K Cooney; G E Kenny
Journal: J Clin Microbiol Date: 1977-02 Impact factor: 5.948

Enhancement of rhinovirus plaque formation in human heteroploid cell cultures by magnesium and calcium.

1. A DESCRIPTION OF TWO NEWLY-RECOGNIZED RHINOVIRUSES OF HUMAN ORIGIN.

2. THE BASIS FOR THE SIZE DIFFERENCES IN PLAQUES PRODUCED BY VARIANTS OF ENCEPHALOMYOCARDITIS (EMC) VIRUS.

3. Magnesium chloride enhancement of cell susceptibility to poliovirus.

4. Serotypes of viruses (rhinoviruses) isolated from common colds.

5. Serological studies on some viruses isolated from common colds (rhinoviruses).

6. An agar polysaccharide and d marker of poliovirus.

7. A plaque method for assaying some viruses isolated from common colds.

8. Improved method for staining cell monolayers for virus plaque counts.

9. A Case of Temporal Arteritis (Horton-Magath Syndrome).

10. Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin.

1. Temperature-dependent innate defense against the common cold virus limits viral replication at warm temperature in mouse airway cells.

2. Comparative susceptibilities of human embryonic fibroblasts and HeLa cells for isolation of human rhinoviruses.

3. Attenuation of rhinovirus type 15: relation of illness to plaque size.

4. An improved in vitro assay for cell-free Marek's disease virus. Brief report.

5. Comparative poliovirus permeability of silver, polycarbonate, and cellulose membrane filters.

6. Selection of variants of rhinoviruses.

7. Plaque formation by 55 rhinovirus serotypes.

8. Rhinovirus plaque formation in WI-38 cells with methylcellulose overlay.

9. Enhancement of the infectivity of rhinovirus ribonucleic acid by diethylaminoethyl dextran.

10. Demonstration of dual rhinovirus infection in humans by isolation of different serotypes in human heteroploid (HeLa) and human diploid fibroblast cell cultures.