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Literature DB >> 4287074

Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin.

C Wallis, F Morales, J Powell, J L Melnick.

Abstract

Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Fred Morales, Joycelyn Powell, and Joseph L. Melnick. Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin. J. Bacteriol. 91:1932-1935. 1966.-Plaque formation of 21 echoviruses (types 1-6, 9, 13, 15-19, 23-26, 29-32) and 8 coxsackieviruses (B1-6, A7, and A9) was enhanced by increased concentrations of MgCl(2), l-cysteine, and pancreatin in agar overlay medium. In most cases, cationic and anionic polymers (diethylaminoethyl dextran, dextran sulfate, or protamine sulfate) were ineffective. All strains of poliovirus and group B coxsackieviruses were enhanced under agar by MgCl(2). Five of the eight coxsackieviruses tested were also enhanced by cysteine or pancreatin. Certain enteroviruses, which have been difficult to assay by plaque method, can now be quantified effectively by incorporation of additives such as MgCl(2), cysteine, or pancreatin into the overlay medium.

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Year: 1966 PMID： 4287074 PMCID： PMC316147 DOI： 10.1128/jb.91.5.1932-1935.1966

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

17 in total

1. DEXTRAN SULFATE PLAQUE VARIANTS OF ATTENUATED TYPE 1 POLIOVIRUS. RELATIONSHIP TO IN VITRO AND IN VIVO MARKERS.

Authors: K K TAKEMOTO; R L KIRSCHSTEIN
Journal: J Immunol Date: 1964-02 Impact factor: 5.422

2. THE BASIS FOR THE SIZE DIFFERENCES IN PLAQUES PRODUCED BY VARIANTS OF ENCEPHALOMYOCARDITIS (EMC) VIRUS.

Authors: H LIEBHABER; K K TAKEMOTO
Journal: Virology Date: 1963-08 Impact factor: 3.616

3. Heterogeneity of Coxsackie B4 virus: two kinds of particles which differ in antibody sensitivity, growth rate, and plaque size.

Authors: P W CHOPPIN; H J EGGERS
Journal: Virology Date: 1962-11 Impact factor: 3.616

2. The enhancing effect of copper, nickel, and cobalt ions on plaque formation by Semliki Forest virus (SFV) in chicken embryo fibroblasts.

Authors: R Wyler; W Wiesendanger
Journal: Arch Virol Date: 1975 Impact factor: 2.574

3. Polioviruses, coxsackieviruses, and echoviruses comparison of the genomes by RNA hybridization.

Authors: N A Young
Journal: J Virol Date: 1973-06 Impact factor: 5.103

4. An improved in vitro assay for cell-free Marek's disease virus. Brief report.

Authors: H K Adldinger; B W Calnek
Journal: Arch Gesamte Virusforsch Date: 1971

5. Trypsin action on the growth of Sendai virus in tissue culture cells. I. Restoration of the infectivity for L cells by direct action of tyrpsin on L cell-borne Sendai virus.

Authors: M Homma
Journal: J Virol Date: 1971-11 Impact factor: 5.103

9. Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction.

Authors: C C Kuo; S P Wang; J T Grayston
Journal: Infect Immun Date: 1973-07 Impact factor: 3.441

10. Enhancement of rhinovirus plaque formation in human heteroploid cell cultures by magnesium and calcium.

Authors: M Fiala; G E Kenny
Journal: J Bacteriol Date: 1966-12 Impact factor: 3.490

Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin.

1. DEXTRAN SULFATE PLAQUE VARIANTS OF ATTENUATED TYPE 1 POLIOVIRUS. RELATIONSHIP TO IN VITRO AND IN VIVO MARKERS.

2. THE BASIS FOR THE SIZE DIFFERENCES IN PLAQUES PRODUCED BY VARIANTS OF ENCEPHALOMYOCARDITIS (EMC) VIRUS.

3. Heterogeneity of Coxsackie B4 virus: two kinds of particles which differ in antibody sensitivity, growth rate, and plaque size.

4. Suppression of adventitious agents in monkey kidney cultures.

5. Magnesium chloride enhancement of cell susceptibility to poliovirus.

6. Plaque formation by myxovirus parainfluenza type 2 in grivet monkey kidney cells.

7. Inhibition of infectious and hemagglutinating properties of type 2 dengue virus by aqueous Agar extracts.

8. Factors influencing enterovirus and reovirus growth and plaque formation.

9. Virus-polysaccharide interactions. I. An agar polysaccharide determining plaque morphology of EMC virus.

10. Mutation of polioviruses with respect to size of plaque. II. Reverse mutation of minute plaque mutant.

1. A comparative study of agar overlay and standard tissue culture methods for isolation of enteroviruses.

2. The enhancing effect of copper, nickel, and cobalt ions on plaque formation by Semliki Forest virus (SFV) in chicken embryo fibroblasts.

3. Polioviruses, coxsackieviruses, and echoviruses comparison of the genomes by RNA hybridization.

4. An improved in vitro assay for cell-free Marek's disease virus. Brief report.

5. Trypsin action on the growth of Sendai virus in tissue culture cells. I. Restoration of the infectivity for L cells by direct action of tyrpsin on L cell-borne Sendai virus.

6. [Antiviral activity of imidazole derivatives. IV. Sensitivity of different viruses].

7. Viral inactivation by disulfide bond reducing agents.

8. Ultraviolet devitalization of eight selected enteric viruses in estuarine water.

9. Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction.

10. Enhancement of rhinovirus plaque formation in human heteroploid cell cultures by magnesium and calcium.