Literature DB >> 4286249

Continuous cultivation of virus in cell suspensions by use of the lysostat.

G B Gori.   

Abstract

Theoretical considerations illustrate the feasibility and characteristics of continuous virus cultivation in cell suspensions Physical dimensions of the virus fermentor (lysostat) in relation to optimal yields can be predetermined. Poliovirus type 1 was grown on Hela S-3-1 (Saltzman) cells in a single-stage, percolator-type lysostat, with a yield of 421 TCID(50) per cell. Adenovirus type 14 was grown on KB (Eagle) cells in a two-stage lysostat with a yield of 116 TCID(50) per cell.

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Mesh：

Year:  1965        PMID： 4286249      PMCID： PMC1058370          DOI： 10.1128/am.13.6.909-917.1965

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  6 in total

1.  Amino acid metabolism in mammalian cell cultures.

Authors:  H EAGLE
Journal:  Science       Date:  1959-08-21       Impact factor: 47.728

2.  THERMAL AND PH STABILITY OF ADENOVIRUS TYPES 12, 14, AND 18.

Authors:  R R RAFAJKO; J C YOUNG
Journal:  Proc Soc Exp Biol Med       Date:  1964-07

3.  TRYPSINIZATION OF ANIMAL TISSUES FOR CELL CULTURE: THEORETICAL CONSIDERATIONS AND AUTOMATIC APPARATUS.

Authors:  G B GORI
Journal:  Appl Microbiol       Date:  1964-03

4.  CHEMOSTATIC CONCENTRATED CULTURES OF HETEROPLOID MAMMALIAN CELL SUSPENSIONS IN DIALYZING FERMENTORS. I. EXPERIMENTAL EVIDENCE AND THEORETICAL CONSIDERATIONS.

Authors:  G B GORI
Journal:  Appl Microbiol       Date:  1965-01

5.  Studies of the biosynthesis of protein and ribonucleic acid in HeLa cells infected with poliovirus.

Authors:  W W ACKERMANN; P C LOH; F E PAYNE
Journal:  Virology       Date:  1959-02       Impact factor: 3.616

6.  The growth cycle of poliovirus in monkey kidney cells. I. Maturation and release of virus in monolayer cultures.

Authors:  D W HOWES; J L MELNICK
Journal:  Virology       Date:  1957-08       Impact factor: 3.616
  6 in total
  5 in total

Review 1.  Dialysis culture of microorganisms: design, theory, and results.

Authors:  J S Schultz; P Gerhardt
Journal:  Bacteriol Rev       Date:  1969-03

Review 2.  Continuous cultivation of microorganisms. A review.

Authors:  I Málek; J Ricica
Journal:  Folia Microbiol (Praha)       Date:  1966       Impact factor: 2.099

Review 3.  Bioreactors for high cell density and continuous multi-stage cultivations: options for process intensification in cell culture-based viral vaccine production.

Authors:  Felipe Tapia; Daniel Vázquez-Ramírez; Yvonne Genzel; Udo Reichl
Journal:  Appl Microbiol Biotechnol       Date:  2016-01-13       Impact factor: 4.813

4.  Efficient and stable production of Modified Vaccinia Ankara virus in two-stage semi-continuous and in continuous stirred tank cultivation systems.

Authors:  Felipe Tapia; Ingo Jordan; Yvonne Genzel; Udo Reichl
Journal:  PLoS One       Date:  2017-08-24       Impact factor: 3.240

5.  Continuous influenza virus production in a tubular bioreactor system provides stable titers and avoids the "von Magnus effect".

Authors:  Felipe Tapia; Daniel Wohlfarth; Volker Sandig; Ingo Jordan; Yvonne Genzel; Udo Reichl
Journal:  PLoS One       Date:  2019-11-05       Impact factor: 3.240
  5 in total

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