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Literature DB >> 14131358

TRYPSINIZATION OF ANIMAL TISSUES FOR CELL CULTURE: THEORETICAL CONSIDERATIONS AND AUTOMATIC APPARATUS.

Abstract

The theoretical background and the experimental feasibility of an automatic control for the continuous trypsinization process are discussed. An automatic apparatus is described with experimental evidence that the optimal mean residence time of monkey kidney cells in the trypsinization flask is between 5 and 8 min, in a volume of fluid approximately ten times that of the tissue processed. A temperature of 36 C and a pH of 7.8 provide optimal conditions for cell viability.

Keywords: CELL NUCLEUS; EQUIPMENT AND SUPPLIES; EXPERIMENTAL LAB STUDY; HYDROGEN-ION CONCENTRATION; KIDNEY; MONKEYS; TISSUE CULTURE; TRYPSIN

Mesh：

Substances：
Trypsin

Year: 1964 PMID： 14131358 PMCID： PMC1058079 DOI： 10.1128/am.12.2.115-121.1964

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

6 in total

1. Stabilized suspension of monkey kidney cells suitable for intercontinental shipment.

Authors: J L MELNICK; C RAPPAPORT; D D BANKER; P N BHATT
Journal: Proc Soc Exp Biol Med Date: 1955-04

2. An apparatus for producing trypsinized monkey kidney cell suspensions.

Authors: L W BISHOP; M K SMITH; A J BEALE
Journal: Virology Date: 1960-02 Impact factor: 3.616

3. [Improved model of an apparatus for trysinization of tissues for cell cultures].

Authors: G BARSKI
Journal: Ann Inst Pasteur (Paris) Date: 1956-07

4. Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells.

Authors: J S YOUNGNER
Journal: Proc Soc Exp Biol Med Date: 1954-02

5. Experiments with the Chemostat on spontaneous mutations of bacteria.

Authors: A NOVICK; L SZILARD
Journal: Proc Natl Acad Sci U S A Date: 1950-12 Impact factor: 11.205

6. Plaque formation and isolation of pure lines with poliomyelitis viruses.

Authors: R DULBECCO; M VOGT
Journal: J Exp Med Date: 1954-02 Impact factor: 14.307

6 in total

4 in total

TRYPSINIZATION OF ANIMAL TISSUES FOR CELL CULTURE: THEORETICAL CONSIDERATIONS AND AUTOMATIC APPARATUS.

1. Stabilized suspension of monkey kidney cells suitable for intercontinental shipment.

2. An apparatus for producing trypsinized monkey kidney cell suspensions.

3. [Improved model of an apparatus for trysinization of tissues for cell cultures].

4. Monolayer tissue cultures. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells.

5. Experiments with the Chemostat on spontaneous mutations of bacteria.

6. Plaque formation and isolation of pure lines with poliomyelitis viruses.

1. Hypoxia abrogates antichlamydial properties of IFN-γ in human fallopian tube cells in vitro and ex vivo.

2. Continuous cultivation of virus in cell suspensions by use of the lysostat.

Review 3. Continuous cultivation of microorganisms. A review.

4. Automatic device for the trypsinization of animal tissues.