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Literature DB >> 4270109

The alkaline cleavage and borohydride reduction of cartilage proteoglycan.

Abstract

A method for the rapid isolation and purification of proteoglycan by using neutral solutions of LiBr for extraction and density-gradient centrifugation is described. The effect of 0.5m-KOH on isolated proteoglycan has been studied by using NaB(3)H(4) to reduce and label the chondroitin sulphate chains released. This study has established: (a) that at least 95% of the chondroitin sulphate chains are attached to the proteoglycan by alkali-labile bonds between xylose and serine; (b) that random degradation of the chondroitin sulphate chains does not occur to any significant extent; (c) that the method is convenient for the determination of polysaccharide number-average molecular weights.

Entities: Chemical Disease

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Year: 1973 PMID： 4270109 PMCID： PMC1177724 DOI： 10.1042/bj1330457

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

23 in total

1. [Studies of the chemistry of the arterial wall. 8. Demonstration, purification and properties of hyaluronidase from the aorta of cattle].

Authors: E Buddecke; D Platt
Journal: Hoppe Seylers Z Physiol Chem Date: 1965

2. Immunochemical studies on blood groups. XXXIV. Structures of some oligosaccharides produced by alkaline degradation of blood group A, B, and H substances.

Authors: K O Lloyd; E A Kabat; E J Layug; F Gruezo
Journal: Biochemistry Date: 1966-05 Impact factor: 3.162

3. [Study on glycoproteins. Demonstration of the O-seryl and O-threonyl-N-acetylgalactosaminidic bonds in the gamma A globulins of human milk].

Authors: J Descamps; M Monsigny; J Montreuil
Journal: C R Acad Hebd Seances Acad Sci D Date: 1968-04-22

4. Physical characterization of dermatan sulphate-protein.

Authors: B N Preston
Journal: Arch Biochem Biophys Date: 1968-09-10 Impact factor: 4.013

Review 5. The determination of molecular weights of biological macromolecules by ultracentrifuge methods.

Authors: J M Creeth; R H Pain
Journal: Prog Biophys Mol Biol Date: 1967 Impact factor: 3.667

6. Studies on porcine costal cartilage protein-polysaccharide complex. II. Chemical studies.

Authors: N Katsura; E A Davidson
Journal: Biochim Biophys Acta Date: 1966-05-26

7. The separation of new forms of the proteinpolysaccharides of bovine nasal cartilage.

Authors: S Pal; P T Doganges; M Schubert
Journal: J Biol Chem Date: 1966-09-25 Impact factor: 5.157

8. The composition and physicochemical properties of bovine nasal-septa protein-polysaccharide complex.

Authors: M Luscombe; C F Phelps
Journal: Biochem J Date: 1967-01 Impact factor: 3.857

9. The simultaneous estimation of 6-deoxy-L-galactose (L-fucose), D-mannose, D-galactose, 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine) and N-acetylneuraminic acid (sialic acid) in glycopeptides and glycoproteins.

Authors: J R Clamp; G Dawson; L Hough
Journal: Biochim Biophys Acta Date: 1967-11-28

10. The structure of a glycopeptide isolated from the yeast cell wall.

Authors: R Sentandreu; D H Northcote
Journal: Biochem J Date: 1968-09 Impact factor: 3.857

15 in total

1. Constant and variable domains of different disaccharide structure in corneal keratan sulphate chains.

Authors: M Oeben; R Keller; H W Stuhlsatz; H Greiling
Journal: Biochem J Date: 1987-11-15 Impact factor: 3.857

Review 2. Proteoglycans of cartilage.

Authors: H Muir
Journal: J Clin Pathol Suppl (R Coll Pathol) Date: 1978

The alkaline cleavage and borohydride reduction of cartilage proteoglycan.

1. [Studies of the chemistry of the arterial wall. 8. Demonstration, purification and properties of hyaluronidase from the aorta of cattle].

2. Immunochemical studies on blood groups. XXXIV. Structures of some oligosaccharides produced by alkaline degradation of blood group A, B, and H substances.

3. [Study on glycoproteins. Demonstration of the O-seryl and O-threonyl-N-acetylgalactosaminidic bonds in the gamma A globulins of human milk].

4. Physical characterization of dermatan sulphate-protein.

Review 5. The determination of molecular weights of biological macromolecules by ultracentrifuge methods.

6. Studies on porcine costal cartilage protein-polysaccharide complex. II. Chemical studies.

7. The separation of new forms of the proteinpolysaccharides of bovine nasal cartilage.

8. The composition and physicochemical properties of bovine nasal-septa protein-polysaccharide complex.

9. The simultaneous estimation of 6-deoxy-L-galactose (L-fucose), D-mannose, D-galactose, 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine) and N-acetylneuraminic acid (sialic acid) in glycopeptides and glycoproteins.

10. The structure of a glycopeptide isolated from the yeast cell wall.

1. Constant and variable domains of different disaccharide structure in corneal keratan sulphate chains.

Review 2. Proteoglycans of cartilage.

3. The synthesis of hyaluronic acid by sheep and rabbit articular cartilage in vitro.

4. The structure and composition of cartilage keratan sulphate.

5. The alkali-labile linkage between keratan sulphate and protein.

6. The chemical constitution of the proteoglycan of human intervertebral disc.

7. Effect of cycloheximide, beta-D-xylosides and beta-D-galactosides on heparin biosynthesis in mouse mastocytoma.

8. N-sulfation of heparan sulfate is critical for syndecan-4-mediated podocyte cell-matrix interactions.

9. The molecular-weight distribution of glycosaminoglycans.

10. The separation and characterization of bronchial glycoproteins by density-gradient methods.