Literature DB >> 4204955

Use of affinity chromatography for the quantitative study of acceptor-ligand interactions: The lactose synthetase system.

P Andrews, B J Kitchen, D J Winzor.   

Abstract

From the effects of N-acetyl-d-glucosamine and d-glucose on the elution of the A protein of human lactose synthetase from a column of Sepharose-alpha-lactalbumin, values of 200m(-1) and 0.57m(-1) are deduced for the association constants describing the interaction between the enzyme and the respective monosaccharides.

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Year:  1973        PMID： 4204955      PMCID： PMC1165911          DOI： 10.1042/bj1350897

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  12 in total

1.  Purification of lactose synthetase a protein from human milk and demonstration of its interaction with alpha-lactalbumin.

Authors:  P Andrews
Journal:  FEBS Lett       Date:  1970-08-31       Impact factor: 4.124

Review 2.  Affinity chromatography.

Authors:  P Cuatrecasas
Journal:  Annu Rev Biochem       Date:  1971       Impact factor: 23.643

3.  The purification and properties of the A protein of lactose synthetase.

Authors:  I P Trayer; R L Hill
Journal:  J Biol Chem       Date:  1971-11       Impact factor: 5.157

4.  Studies on galactosyltransferase. Kinetic investigations with glucose as the galactosyl group acceptor.

Authors:  J F Morrison; K E Ebner
Journal:  J Biol Chem       Date:  1971-06-25       Impact factor: 5.157

5.  The structure, function, and evolution of alpha-lactalbumin.

Authors:  R L Hill; K Brew; T C Vanaman; I P Trayer; P Mattock
Journal:  Brookhaven Symp Biol       Date:  1968-06

6.  Alpha-lactalbumin and the lactose synthetase reaction.

Authors:  D K Fitzgerald; U Brodbeck; I Kiyosawa; R Mawal; B Colvin; K E Ebner
Journal:  J Biol Chem       Date:  1970-04-25       Impact factor: 5.157

7.  The role of alpha-lactalbumin in lactose synthetase.

Authors:  W A Klee; C B Klee
Journal:  Biochem Biophys Res Commun       Date:  1970-06-05       Impact factor: 3.575

8.  Purification and properties of bovine milk glyco-alpha-lactalbumin.

Authors:  T E Barman
Journal:  Biochim Biophys Acta       Date:  1970-07-27

9.  Studies on galactosyltransferase. Detection of enzyme-reactant complexes by means of affinity chromatography.

Authors:  R Mawal; J F Morrison; K E Ebner
Journal:  J Biol Chem       Date:  1971-12-10       Impact factor: 5.157

10.  Resolution of a soluble lactose synthetase into two protein components and solubilization of microsomal lactose synthetase.

Authors:  U Brodbeck; K E Ebner
Journal:  J Biol Chem       Date:  1966-02-10       Impact factor: 5.157
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  7 in total

Review 1.  Analysis of solute-protein interactions and solute-solute competition by zonal elution affinity chromatography.

Authors:  Pingyang Tao; Saumen Poddar; Zuchen Sun; David S Hage; Jianzhong Chen
Journal:  Methods       Date:  2018-02-02       Impact factor: 3.608

2.  Evaluation of equilibrium constants by affinity chromatography.

Authors:  L W Nichol
Journal:  Biochem J       Date:  1974-11       Impact factor: 3.857

Review 3.  The study of ligand-protein interactions utilizing affinity chromatography.

Authors:  B M Dunn
Journal:  Appl Biochem Biotechnol       Date:  1984-06       Impact factor: 2.926

4.  Evaluation of equilibrium constants for the interaction of lactate dehydrogenase isoenzymes with reduced nicotinamide-adenine dinucleotide by affinity chromatography.

Authors:  R I Brinkworth; C J Masters; D J Winzor
Journal:  Biochem J       Date:  1975-12       Impact factor: 3.857

5.  A new method of quantitative affinity chromatography and its application to the study of myosin.

Authors:  R C Bottomley; A C Storer; I P Trayer
Journal:  Biochem J       Date:  1976-12-01       Impact factor: 3.857

6.  Quantitative affinity chromatography. Determination of binding constants by elution with competitive inhibitors.

Authors:  B M Dunn; I M Chaiken
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

7.  Studies on the thiol group of lactose synthetase A protein from human milk and on the binding of uridine diphosphate galactose to the enzyme.

Authors:  B J Kitchen; P Andrens
Journal:  Biochem J       Date:  1974-07       Impact factor: 3.857
  7 in total

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