Literature DB >> 11947697

Purification of lactose synthetase a protein from human milk and demonstration of its interaction with alpha-lactalbumin.

P Andrews1.   

Abstract

A fairly simple procedure for isolating lactose synthetase A protein from human milk in 36% yield is described. The A protein has a molecular weight of about 40,000. Its interaction with alpha-lactalbumin in the presence of N-acetylglucosamine has been demonstrated by affinity chromatography and gel filtration.

Entities:  

Year:  1970        PMID： 11947697     DOI： 10.1016/0014-5793(70)80382-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  14 in total

1.  Solubilization and purification of galactosyltransferase from golgi membranes of rat ventral prostate.

Authors:  B Tadolini; G Hakim
Journal:  Appl Biochem Biotechnol       Date:  1982-07       Impact factor: 2.926

2.  UDP-N-Acetyl-alpha-D-glucosamine as acceptor substrate of beta-1,4-galactosyltransferase. Enzymatic synthesis of UDP-N-acetyllactosamine.

Authors:  L Elling; A Zervosen; R G Gallego; V Nieder; M Malissard; E G Berger; J F Vliegenthart; J P Kamerling
Journal:  Glycoconj J       Date:  1999-07       Impact factor: 2.916

3.  Possible role of a thiol group in lactose synthetase A protein.

Authors:  B J Kitchen; P Andrews
Journal:  Biochem J       Date:  1972-11       Impact factor: 3.857

4.  Use of affinity chromatography for the quantitative study of acceptor-ligand interactions: The lactose synthetase system.

Authors:  P Andrews; B J Kitchen; D J Winzor
Journal:  Biochem J       Date:  1973-12       Impact factor: 3.857

5.  Affinity-chromatography studies on the A protein of lactose synthetase.

Authors:  I P Trayer; K W Olsen
Journal:  Biochem J       Date:  1972-04       Impact factor: 3.857

6.  Rat liver Golgi galactosyltransferases. Distinct enzymes for glycolipid and glycoprotein acceptor substrates.

Authors:  F Kaplan; P Hechtman
Journal:  Biochem J       Date:  1984-01-15       Impact factor: 3.857

7.  Studies on the purification and properties of UDP-galactose glycoprotein galactosyltransferase from rat liver and serum.

Authors:  I H Fraser; S Mookerjea
Journal:  Biochem J       Date:  1976-05-15       Impact factor: 3.857

8.  Hen ovomucoid-agarose: A new conjugate for the isolation by affinity chromatography of UDP-galactose: Glycoprotein galactosyl-transferase.

Authors:  B Tadolini; G Hakim
Journal:  Appl Biochem Biotechnol       Date:  1981-09       Impact factor: 2.926

9.  Studies on the thiol group of lactose synthetase A protein from human milk and on the binding of uridine diphosphate galactose to the enzyme.

Authors:  B J Kitchen; P Andrens
Journal:  Biochem J       Date:  1974-07       Impact factor: 3.857

10.  Human plasma uridine diphosphate galactose-glycoprotein galactosyltransfertase. Purification, properties and kinetics of the enzyme-catalysed reaction.

Authors:  A Bella; J S Whitehead; Y S Kim
Journal:  Biochem J       Date:  1977-12-01       Impact factor: 3.857
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