Literature DB >> 4200727

Relation of detergent HLB number to solubilization and stabilization of D-alanine carboxypeptidase from Bacillus subtilis membranes.

J N Umbreit, J L Strominger.   

Abstract

The ability of various nonionic detergents to solubilize D-alanine carboxypeptidase and other membrane-bound enzymes was correlated to a physical property of the detergent, the HLB number. Only a fairly narrow range of detergents were effective solubilizing agents. Purified carboxypeptidase required either a detergent or detergent plus a lipid fraction for stability. Only those detergents effective in solubilizing the enzyme were effective in stabilizing it.

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Year:  1973        PMID： 4200727      PMCID： PMC427155          DOI： 10.1073/pnas.70.10.2997

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  3 in total

1.  A rapid method of total lipid extraction and purification.

Authors:  E G BLIGH; W J DYER
Journal:  Can J Biochem Physiol       Date:  1959-08

2.  Structure and synthesis of a lipid-containing bacteriophage. II. Alterations in cytoplasmic and membrane-bound enzymes during replication of bacteriophage PM2.

Authors:  A Datta; R M Franklin
Journal:  Virology       Date:  1969-11       Impact factor: 3.616

3.  Complex lipid requirements for detergent-solubilized phosphoacetylmuramyl-pentapeptide translocase from Micrococcus luteus.

Authors:  J N Umbreit; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205
  3 in total
  14 in total

1.  Peptidoglycan biosynthesis in Micrococcus luteus (sodonensis): transglycosidase and phosphodiesterase activities in membrane preparations.

Authors:  S E Jensen; J N Campbell
Journal:  J Bacteriol       Date:  1976-07       Impact factor: 3.490

2.  Amidase activity involved in peptidoglycan biosynthesis in membranes of Micrococcus luteus (sodonensis).

Authors:  S E Jensen; J N Campbell
Journal:  J Bacteriol       Date:  1976-07       Impact factor: 3.490

3.  A reagent to facilitate protein recovery from cells and tissues.

Authors:  Byeong Hee Hwang; Nishit Doshi; Kenneth Y Tsai; Samir Mitragotri
Journal:  Drug Deliv Transl Res       Date:  2012-10       Impact factor: 4.617

Review 4.  Interaction of penicillin with the bacterial cell: penicillin-binding proteins and penicillin-sensitive enzymes.

Authors:  P M Blumberg; J L Strominger
Journal:  Bacteriol Rev       Date:  1974-09

5.  Purification and properties of Escherichia coli dimethyl sulfoxide reductase, an iron-sulfur molybdoenzyme with broad substrate specificity.

Authors:  J H Weiner; D P MacIsaac; R E Bishop; P T Bilous
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

6.  Cellular lysis of Streptococcus faecalis induced with triton X-100.

Authors:  J B Cornett; G D Shockman
Journal:  J Bacteriol       Date:  1978-07       Impact factor: 3.490

7.  Relating surfactant properties to activity and solubilization of the human adenosine a3 receptor.

Authors:  Bryan W Berger; Roxana Y García; Abraham M Lenhoff; Eric W Kaler; Clifford R Robinson
Journal:  Biophys J       Date:  2005-04-22       Impact factor: 4.033

8.  Use of resistant mutants to study the interaction of triton X-100 with Staphylococcus aureus.

Authors:  D Raychaudhuri; A N Chatterjee
Journal:  J Bacteriol       Date:  1985-12       Impact factor: 3.490

9.  Tripod Amphiphiles for Membrane Protein Manipulation.

Authors:  Pil Seok Chae; Philip D Laible; Samuel H Gellman
Journal:  Mol Biosyst       Date:  2010

10.  Effect of non-lytic concentrations of Brij series detergents on the metabolism-independent ion permeability properties of human erythrocytes.

Authors:  A Miseta; P Bogner; A Szarka; M Kellermayer; C Galambos; D N Wheatley; I L Cameron
Journal:  Biophys J       Date:  1995-12       Impact factor: 4.033
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