Literature DB >> 417050

An efficient method to produce specific anti-actin.

B M Jockusch, K H Kelley, R K Meyer, M M Burger.   

Abstract

The production of affinity column purified (specific) anti-actin is described. With the immunization scheme employed, all rabbits produced precipitating antibodies over several months, so that 30 mg specific anti-actin per rabbit could be isolated in 6 months. The antibodies against native and detergent denatured smooth muscle actin are characterized by immunodiffusion tests, staining of the I-band of isolated myofibrils and stress fibers in tissue culture cells, using indirect immunofluorescence.

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Year:  1978        PMID： 417050     DOI： 10.1007/bf00495757

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  25 in total

1.  Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin.

Authors:  G M Fuller; B R Brinkley; J M Boughter
Journal:  Science       Date:  1975-03-14       Impact factor: 47.728

2.  Head to tail polymerization of actin.

Authors:  A Wegner
Journal:  J Mol Biol       Date:  1976-11       Impact factor: 5.469

3.  ATP binding to a protease-resistant core of actin.

Authors:  G R Jacobson; J P Rosenbusch
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

4.  Immunofluorescent tracing of smooth muscle contractile protein antigens in tissues other than smooth muscle.

Authors:  P Trenchev; P Sneyd; E J Holborow
Journal:  Clin Exp Immunol       Date:  1974-01       Impact factor: 4.330

5.  Complete amino-acid sequence of actin of rabbit skeletal muscle.

Authors:  M Elzinga; J H Collins; W M Kuehl; R S Adelstein
Journal:  Proc Natl Acad Sci U S A       Date:  1973-09       Impact factor: 11.205

6.  Comparison of vascular smooth muscle cells from adult human, monkey and rabbit in primary culture and in subculture.

Authors:  J H Chamley; G R Campbell; J D McConnell; U Gröschel-Stewart
Journal:  Cell Tissue Res       Date:  1977-02-14       Impact factor: 5.249

7.  Studies on the isolation and molecular properties of homogeneous globular actin. Evidence for a single polypeptide chain structure.

Authors:  M K Rees; M Young
Journal:  J Biol Chem       Date:  1967-10-10       Impact factor: 5.157

8.  Fixation of human anti-actin autoantibodies on skeletal muscle fibres.

Authors:  C Chaponnier; L Kohler; G Gabbiani
Journal:  Clin Exp Immunol       Date:  1977-02       Impact factor: 4.330

9.  Actin is the naturally occurring inhibitor of deoxyribonuclease I.

Authors:  E Lazarides; U Lindberg
Journal:  Proc Natl Acad Sci U S A       Date:  1974-12       Impact factor: 11.205

10.  A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence.

Authors:  W Z Cande; E Lazarides; J R McIntosh
Journal:  J Cell Biol       Date:  1977-03       Impact factor: 10.539
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  33 in total

1.  Microfilament-rich cells in the toad bladder epithelium.

Authors:  J P Kraehenbuhl; J Pfeiffer; M Rossier; B C Rossier
Journal:  J Membr Biol       Date:  1979-07-16       Impact factor: 1.843

2.  Tissue specific nuclear antigens in the germinal vesicle ofXenopus laevis oocytes.

Authors:  Christine Dreyer; Helen Singer; Peter Hausen; Ursula Müller; Elisabeth Siegel
Journal:  Wilehm Roux Arch Dev Biol       Date:  1981-07

3.  Fine structure and immunofluorescent studies of the WISH cell line.

Authors:  W D Meek; W L Davis
Journal:  In Vitro Cell Dev Biol       Date:  1986-12

4.  Tumor dedifferentiation: an important step in tumor invasion.

Authors:  H Gabbert; R Wagner; R Moll; C D Gerharz
Journal:  Clin Exp Metastasis       Date:  1985 Oct-Dec       Impact factor: 5.150

5.  Immunological analysis of nuclear lamina proteins.

Authors:  R Stick; P Hausen
Journal:  Chromosoma       Date:  1980       Impact factor: 4.316

6.  Studies on the antigenic sites of actin: a comparative study of the immunogenic crossreactivity of invertebrate actins.

Authors:  H G De Couet
Journal:  J Muscle Res Cell Motil       Date:  1983-08       Impact factor: 2.698

7.  Appearance of cytoskeletal components on the surface of leukemia cells and of lymphocytes transformed by mitogens and Epstein-Barr virus.

Authors:  R J Bachvaroff; F Miller; F T Rapaport
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

8.  Actin distribution and synthesis in human fibroblasts infected by cytomegalovirus.

Authors:  D Lösse; R Lauer; D Weder; K Radsak
Journal:  Arch Virol       Date:  1982       Impact factor: 2.574

9.  A monoclonal antibody that detects vimentin-related proteins in invertebrates.

Authors:  M F Walter; H Biessmann
Journal:  Mol Cell Biochem       Date:  1984       Impact factor: 3.396

10.  The 180-kD component of the neural cell adhesion molecule N-CAM is involved in cell-cell contacts and cytoskeleton-membrane interactions.

Authors:  G E Pollerberg; K Burridge; K E Krebs; S R Goodman; M Schachner
Journal:  Cell Tissue Res       Date:  1987-10       Impact factor: 5.249
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