Literature DB >> 1096300

Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin.

G M Fuller, B R Brinkley, J M Boughter.   

Abstract

Monospecific antibody directed against bovine brain tubulin has been purified by affinity chromatography and tested against soluble tubulin and intact microtubules of brain and mitotic apparatus. Binding of the tubulin antibody to the mitotic spindle of rat kangaroo cells was demonstrated in all stages of mitosis by indirect immunofluorescence.

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Year:  1975        PMID： 1096300     DOI： 10.1126/science.1096300

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  67 in total

1.  Increased expression of a 58-kDa protein kinase leads to changes in the CHO cell cycle.

Authors:  B A Bunnell; L S Heath; D E Adams; J M Lahti; V J Kidd
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

2.  Control of microtubule assembly-disassembly by calcium-dependent regulator protein.

Authors:  J M Marcum; J R Dedman; B R Brinkley; A R Means
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

3.  Cytoplasmic microtubules in tissue culture cells appear to grow from an organizing structure towards the plasma membrane.

Authors:  M Osborn; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

4.  Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head.

Authors:  P Dráber; E Dráberová; V Viklický
Journal:  Histochemistry       Date:  1991

5.  Regulation of tubulin synthesis in islets of Langerhans.

Authors:  D G Pipeleers; M A Pipeleers-Marichal; D M Kipnis
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

6.  Calcium-dependent regulator protein: localization in mitotic apparatus of eukaryotic cells.

Authors:  M J Welsh; J R Dedman; B R Brinkley; A R Means
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

7.  [Immunofluorescence shows cell structure].

Authors:  E Schnepf
Journal:  Naturwissenschaften       Date:  1977-02

8.  Localization of tubulin in the mitotic apparatus of mammalian cells by immunofluorescence and immunoelectron microscopy.

Authors:  D A Pepper; B R Brinkley
Journal:  Chromosoma       Date:  1977-04-19       Impact factor: 4.316

9.  Immunoflourescent staining of cytoplasmic and spindle microtubules in mouse fibroblasts with antibody to tau protein.

Authors:  J A Connolly; V I Kalnins; D W Cleveland; M W Kirschner
Journal:  Proc Natl Acad Sci U S A       Date:  1977-06       Impact factor: 11.205

10.  Organization and energy-dependent growth of microtubules in cells.

Authors:  F R Frankel
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205
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