Literature DB >> 41650

A new assay for diaphorase activity in reagent formulations, based on the reduction of thiazolyl blue.

R S Boethling, T L Weaver.   

Abstract

This new assay procedure for diaphorase eliminates problems of high blank rates and nonlinear kinetics associated with other methods. The dye thiazolyl blue tetrazolium bromide is reduced in the presence of NADH and diaphorase to yield a colored formazan, which as maximum absorbance at 560 nm.

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Year:  1979        PMID: 41650

Source DB:  PubMed          Journal:  Clin Chem        ISSN: 0009-9147            Impact factor:   8.327


  6 in total

1.  Differential roles of nitric oxide synthases in regulation of ultraviolet B light-induced apoptosis.

Authors:  Wei Liu; Shiyong Wu
Journal:  Nitric Oxide       Date:  2010-06-12       Impact factor: 4.427

2.  Recombinant expression, characterization and application of a dihydrolipoamide dehydrogenase with diaphorase activity from Bacillus sphaericus.

Authors:  Anvarsadat Kianmehr; Rahman Mahdizadeh; Morteza Oladnabi; Javad Ansari
Journal:  3 Biotech       Date:  2017-06-08       Impact factor: 2.406

3.  Medium Optimization for Improved Production of Dihydrolipohyl Dehydrogenase from Bacillus sphaericus PAD-91 in Escherichia coli.

Authors:  Hamid Shahbazmohammadi; Eskandar Omidinia
Journal:  Mol Biotechnol       Date:  2017-07       Impact factor: 2.695

Review 4.  Avoiding Fluorescence Assay Interference-The Case for Diaphorase.

Authors:  Matthew D Hall; Anton Simeonov; Mindy I Davis
Journal:  Assay Drug Dev Technol       Date:  2016-04       Impact factor: 1.738

5.  Diaphorase Coupling Protocols for Red-Shifting Dehydrogenase Assays.

Authors:  Mindy I Davis; Min Shen; Anton Simeonov; Matthew D Hall
Journal:  Assay Drug Dev Technol       Date:  2016-04       Impact factor: 1.738

6.  Molecular and functional characterization of ferredoxin NADP(H) oxidoreductase from Gracilaria chilensis and its complex with ferredoxin.

Authors:  María Alejandra Vorphal; Carola Bruna; Traudy Wandersleben; Jorge Dagnino-Leone; Francisco Lobos-González; Elena Uribe; José Martínez-Oyanedel; Marta Bunster
Journal:  Biol Res       Date:  2017-12-08       Impact factor: 5.612

  6 in total

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