Literature DB >> 4149766

Assay for glutamine synthetase activity.

J E Vorhaben, L Wong, J W Campbell.   

Abstract

The use of phosphoenolpyruvate plus pyruvate kinase as an ATP-generating system in the assay for glutamine synthetase activity via the formation of gamma-glutamylhydroxamate from glutamate and hydroxylamine with crude tissue preparations is shown to give values far in excess of the true glutamine synthetase activity of the tissue. This is due to the generation of pyruvate, which reacts with hydroxylamine to give a compound that is chromogenic with the ferric chloride reagent used for measuring gamma-glutamylhydroxamate.

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Year:  1973        PMID： 4149766      PMCID： PMC1165910          DOI： 10.1042/bj1350893

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  6 in total

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Authors:  V PAMILJANS; P R KRISHNASWAMY; G DUMVILLE; A MEISTER
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2.  Studies on the enzymic synthesis of glutamine.

Authors:  W H ELLIOTT
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3.  Glutamine synthetase. A mitochondrial enzyme in uricotelic species.

Authors:  J E Vorhaben; J W Campbell
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4.  Glutamine synthetase activity in tissues of lower vertebrates.

Authors:  P Lund; L Goldstein
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5.  Glutamine synthetase. Determination of its distribution in brain during development.

Authors:  S Berl
Journal:  Biochemistry       Date:  1966-03       Impact factor: 3.162

6.  The distribution between gamma-glutamylhydrozamate synthetase and L-glutamine-hydroxylamine glutamyltransferase activities in rat tissues. Studies in vitro.

Authors:  A Herzfeld
Journal:  Biochem J       Date:  1973-05       Impact factor: 3.857
  6 in total
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Journal:  Biochem J       Date:  1998-03-01       Impact factor: 3.857

2.  Postmortem changes in the enzymes of GABA and glutamate metabolism in the cerebellum and forebrain of newborn and adult rats.

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5.  Submitochondrial localization and function of enzymes of glutamine metabolism in avian liver.

Authors:  J E Vorhaben; J W Campbell
Journal:  J Cell Biol       Date:  1977-05       Impact factor: 10.539

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  6 in total

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