Literature DB >> 414223

Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart.

C Brack, S Tonegawa.   

Abstract

A DNA fragment carrying an immunoglobulin gene coding for both variable (V) and constant (C) regions of a mouse lambda light chain was enriched about 15-fold from a total endonuclease EcoRI digest of a plasmacytoma (HOPC 2020) DNA by preparative agarose gel electrophoresis. The DNA fraction was used for cloning of a lambda chain gene in the phage lambdagtWES vector. After screening [Benton, W. D. & Davis, R. W. (1977) Science 196, 180-182] of about 70,000 plaques, each arising from an independent transfection event, we isolated one clone (Ig 303) that contained both a Vlambda and a Clambda DNA sequence. Electron microscopy of R-loops formed between the cloned DNA and purified lambda chain mRNA (HOPC 2020) revealed that the Vlambda and Clambda DNA sequences are separated by a 1250-base DNA fragment.

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Year:  1977        PMID： 414223      PMCID： PMC431846          DOI： 10.1073/pnas.74.12.5652

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.

Authors:  L T Chow; R E Gelinas; T R Broker; R J Roberts
Journal:  Cell       Date:  1977-09       Impact factor: 41.582

2.  Screening lambdagt recombinant clones by hybridization to single plaques in situ.

Authors:  W D Benton; R W Davis
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

3.  EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

Authors:  P Leder; D Tiemeier; L Enquist
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

4.  Cloning of an immunoglobulin variable region gene from mouse embryo.

Authors:  S Tonegawa; C Brack; N Hozumi; R Schuller
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

5.  Amplification and characterization of a beta-globin gene synthesized in vitro.

Authors:  T Maniatis; S G Kee; A Efstratiadis; F C Kafatos
Journal:  Cell       Date:  1976-06       Impact factor: 41.582

6.  Somatic changes in the content and context of immunoglobulin genes.

Authors:  S Tonegawa; N Hozumi; G Matthyssens; R Schuller
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1977

7.  A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.

Authors:  D M Glover; D S Hogness
Journal:  Cell       Date:  1977-02       Impact factor: 41.582

8.  R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.

Authors:  R L White; D S Hogness
Journal:  Cell       Date:  1977-02       Impact factor: 41.582

Review 9.  The structure and genetics of mouse immunoglobulins: an analysis of NZB myeloma proteins and sets of BALB/c myeloma proteins binding particular haptens.

Authors:  L Hood; E Loh; J Hubert; P Barstad; B Eaton; P Early; J Fuhrman; N Johnson; M Kronenberg; J Schilling
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1977

10.  Spliced segments at the 5' terminus of adenovirus 2 late mRNA.

Authors:  S M Berget; C Moore; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
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  46 in total

1.  Cloning of immunoglobulin kappa light chain genes from mouse liver and myeloma MOPC 173.

Authors:  M Steinmetz; H G Zachau; B Mach
Journal:  Nucleic Acids Res       Date:  1979-07-25       Impact factor: 16.971

2.  DNA clones containing mouse immunoglobulin kappa chain genes isolated by in vitro packaging into phage lambda coats.

Authors:  R Lenhard-Schuller; B Hohn; C Brack; M Hirama; S Tonegawa
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

3.  Variable and constant regions are separated in the 10-kbase transcription unit coding for immunoglobulin kappa light chains.

Authors:  M Gilmore-Herbert; K Hercules; M Komaromy; R Wall
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

Review 4.  Recombinant antibodies for the diagnosis and treatment of cancer.

Authors:  Jürgen Krauss
Journal:  Mol Biotechnol       Date:  2003-09       Impact factor: 2.695

5.  Electron microscopic analysis of transcription: mapping of initiation sites and direction of transcription.

Authors:  C Brack
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

Review 6.  Multiple forms of inducible drug-metabolizing enzymes: a reasonable mechanism by which any organism can cope with adversity.

Authors:  D W Nebert
Journal:  Mol Cell Biochem       Date:  1979-09-28       Impact factor: 3.396

7.  Large and small tumor antigens from simian virus 40 have identical amino termini mapping at 0.65 map units.

Authors:  E Paucha; A Mellor; R Harvey; A E Smith; R M Hewick; M D Waterfield
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205

8.  Characterization of a mouse DNA clone containing an immunoglobulin variable region gene.

Authors:  N Hozumi; C Brack; V Pirrotta; R Lenhard-Schuller; S Tonegawa
Journal:  Nucleic Acids Res       Date:  1978-06       Impact factor: 16.971

Review 9.  Epigenetic aspects of lymphocyte antigen receptor gene rearrangement or 'when stochasticity completes randomness'.

Authors:  Sébastien Jaeger; Bastien Fernandez; Pierre Ferrier
Journal:  Immunology       Date:  2013-06       Impact factor: 7.397

10.  The ovalbumin gene: structural sequences in native chicken DNA are not contiguous.

Authors:  E C Lai; S L Woo; A Dugaiczyk; J F Catterall; B W O'Malley
Journal:  Proc Natl Acad Sci U S A       Date:  1978-05       Impact factor: 11.205
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