Literature DB >> 4128544

Studies on purified eel acetylcholine receptor and anti-acetylcholine receptor antibody.

J Patrick, J Lindstrom, B Culp, J McMillan.   

Abstract

Rabbit antiserum against purified Electrophorus electricus acetylcholine receptor is studied using an immunoprecipitin assay to measure either antibody titer or concentration of toxin-binding sites in solubilized receptor preparations. This antiserum, unlike control serum, blocks the electrophysiological response of the electroplax to carbamylcholine. Toxin (alpha-neurotoxin, Naja naja) and several cholinergic ligands produce partial inhibition of the reaction of antiserum with purified acetylcholine receptor. Evidence is presented that some of the toxin-binding sites on receptor, purified by affinity chromatography on toxin-agarose conjugates, are occluded by toxin. In addition, evidence is presented that antireceptor antiserum will cause precipitation of more toxin-binding sites present in an initial extract than in purified receptor preparations.

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Year:  1973        PMID： 4128544      PMCID： PMC427231          DOI： 10.1073/pnas.70.12.3334

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  Properties of the nicotinic acetylcholine receptor macromolecule of Electrophorus electricus.

Authors:  B Fulpius; S Cha; R Klett; E Reich
Journal:  FEBS Lett       Date:  1972-08-15       Impact factor: 4.124

2.  Isolation of the cholinergic receptor protein of Torpedo electric tissue.

Authors:  R Miledi; P Molinoff; L T Potter
Journal:  Nature       Date:  1971-02-19       Impact factor: 49.962

3.  Autoradiographic detection of antigens in cells using tritium-labeled antibodies.

Authors:  K Ostrowski; E A Barnard; W Sawicki; T Chorzelski; A Langner; A Mikulski
Journal:  J Histochem Cytochem       Date:  1970-07       Impact factor: 2.479

4.  Progress in the purification of the cholinergic receptor protein from Electrophorus electricus by affinity chromatography.

Authors:  R W Olsen; J C Meunier; J P Changeux
Journal:  FEBS Lett       Date:  1972-11-15       Impact factor: 4.124

5.  Use of affinity chromatography for acetylcholine receptor purification.

Authors:  J Schmidt; M A Raftery
Journal:  Biochem Biophys Res Commun       Date:  1972-10-17       Impact factor: 3.575

6.  Isolation of the nicotinic acetylcholine receptor by biospecific chromatography on insolubilized Naja naja neurotoxin.

Authors:  E Karlsson; E Heilbronn; L Widlund
Journal:  FEBS Lett       Date:  1972-11-15       Impact factor: 4.124

7.  [Separation of the receptor protein of acetylcholine and acetylcholinesterase].

Authors:  J C Meunier; M Huchet; P Boquet; J P Changeux
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1971-01-04

8.  Appearance of acetylcholine receptors during differentiation of a myogenic cell line.

Authors:  J Patrick; S F Heinemann; J Lindstrom; D Schubert; J H Steinbach
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205

9.  Characterization and partial purification of the acetylcholine receptor from Torpedo electroplax.

Authors:  M E Eldefrawi; A T Eldefrawi
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205

10.  Binding of -bungarotoxin to acetylcholine receptors in mammalian muscle (snake venom-denervated muscle-neonatal muscle-rat diaphragm-SDS-polyacrylamide gel electrophoresis).

Authors:  D K Berg; R B Kelly; P B Sargent; P Williamson; Z W Hall
Journal:  Proc Natl Acad Sci U S A       Date:  1972-01       Impact factor: 11.205
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  30 in total

1.  Photoaffinity labeling and quaternary structure of the acetylcholine receptor from Torpedo californica.

Authors:  F Hucho; P Layer; H R Kiefer; G Bandini
Journal:  Proc Natl Acad Sci U S A       Date:  1976-08       Impact factor: 11.205

2.  Synthesis of acetylcholine receptor by denervated rat diaphragm muscle.

Authors:  J P Brockes; Z W Hall
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

3.  Editorial: "Myasthenia gravis" or the autoimmunity, thymic disease, neuromuscular block poser.

Authors:  G Goldstein
Journal:  West J Med       Date:  1975-08

4.  A cholinoceptor antiserum: its pharmacological properties.

Authors:  F Berti; F Clementi; B Conti-Tronconi; G C Folco
Journal:  Br J Pharmacol       Date:  1976-05       Impact factor: 8.739

5.  Interactions of antinicotinic acetylcholine receptor antibodies with rat brain and muscle antigenic determinants.

Authors:  R J Lukas
Journal:  Cell Mol Neurobiol       Date:  1986-09       Impact factor: 5.046

6.  Semisynthetic cytochrome c.

Authors:  L E Barstow; R S Young; E Yakali; J J Sharp; J C O'Brien; P W Berman; H A Harbury
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

7.  Immunological distinction between acetylcholine receptor and the alpha-bungarotoxin-binding component on sympathetic neurons.

Authors:  J Patrick; W B Stallcup
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

Review 8.  Myasthenia gravis--current concepts.

Authors:  C Herrmann; J M Lindstrom; J C Keesey; D G Mulder
Journal:  West J Med       Date:  1985-06

9.  Presence of autoantibody for phospholipase inhibitory protein, lipomodulin, in patients with rheumatic diseases.

Authors:  F Hirata; R del Carmine; C A Nelson; J Axelrod; E Schiffmann; A Warabi; A L De Blas; M Nirenberg; V Manganiello; M Vaughan; S Kumagai; I Green; J L Decker; A D Steinberg
Journal:  Proc Natl Acad Sci U S A       Date:  1981-05       Impact factor: 11.205

10.  Acetylcholine receptor and thymus in experimental autoimmune myasthenia gravis and experimental myositis.

Authors:  S Ueno; K Wada; J Kang; M Takahashi; S Tarui
Journal:  Clin Exp Immunol       Date:  1982-12       Impact factor: 4.330
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