Literature DB >> 4117294

Electron microscopic localization of alpha toxin within the staphylococcal cell by ferritin-labeled antibody.

J R Coulter, T M Mukherjee.   

Abstract

Highly purified staphylococcal alpha toxin has been used to produce monospecific anti-alpha antibody in rabbits. Gamma globulin prepared from the serum of these rabbits was coupled with ferritin by using toluene diisocyanate. Staphylococcal cells which had been disrupted by two passages through an LKB X-press were treated with this conjugate. Electron microscopic examination of this material showed alpha toxin or an antigenically mature precursor located on the cytoplasmic membrane. The possible function of alpha toxin in this situation is discussed.

Entities:  

Mesh：

Substances：

Year:  1971        PMID： 4117294      PMCID： PMC416365          DOI： 10.1128/iai.4.5.650-655.1971

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  7 in total

1.  Characteristics of the micrococcal nasal carrier state among hospital personnel.

Authors:  M H LEPPER; G G JACKSON; H F DOWLING
Journal:  J Lab Clin Med       Date:  1955-06

2.  Isolation and composition of staphylococcal alpha toxin.

Authors:  A W BERNHEIMER; L L SCHWARTZ
Journal:  J Gen Microbiol       Date:  1963-03

3.  Virulence and coagulases of Staphylococcus aureus.

Authors:  F A KAPRAL; I W LI
Journal:  Proc Soc Exp Biol Med       Date:  1960-05

4.  Stimulation of staphylococcal haemolysin production by low concentrations of penicillin.

Authors:  H O Hallander; G Laurell; G Löfström
Journal:  Acta Pathol Microbiol Scand       Date:  1966

5.  Electron microscopy and viability of lysostaphin-induced staphylococcal spheroplasts, protoplast-like bodies, and protoplasts.

Authors:  V T Schuhardt; T W Huber; L M Pope
Journal:  J Bacteriol       Date:  1969-01       Impact factor: 3.490

6.  Production, purification, and composition of staphylococcal alpha toxin.

Authors:  J R Coulter
Journal:  J Bacteriol       Date:  1966-12       Impact factor: 3.490

7.  Studies on plasma membranes. IV. The ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma.

Authors:  E L Benedetti; P Emmelot
Journal:  J Cell Sci       Date:  1967-12       Impact factor: 5.285
  7 in total
  5 in total

Review 1.  The hemolysins of Staphylococcus aureus.

Authors:  G M Wiseman
Journal:  Bacteriol Rev       Date:  1975-12

2.  Cellular location of streptolysin O.

Authors:  G B Calandra; T S Theodore
Journal:  Infect Immun       Date:  1975-10       Impact factor: 3.441

3.  Cellular location of alpha-hemolysin in Staphylococcus aureus.

Authors:  K M Nugent; G B Calandra; T S Theodore
Journal:  Infect Immun       Date:  1975-08       Impact factor: 3.441

4.  The demonstration of the existence of an interlayer between the cytoplasmic membrane and the cell wall proper of staphylococci.

Authors:  P Giesbrecht; J Wecke; B Reinicke
Journal:  Arch Microbiol       Date:  1977-10-24       Impact factor: 2.552

5.  Effect of phenethyl alcohol on Staphylococcus aureus alpha-lysin production.

Authors:  K Y Lee; T H Birkbeck
Journal:  Infect Immun       Date:  1985-01       Impact factor: 3.441
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.