Literature DB >> 4091812

A sensitive assay for active link protein from cartilage.

J D Sandy, A H Plaas.   

Abstract

A new assay for the activity of cartilage link protein is described. The method is based on the finding [Plaas, Sandy & Muir (1983) Biochem. J. 214, 855-864] that addition of link protein to [35S]sulphate-labelled proteoglycan aggregates from rabbit chondrocyte cultures resulted in the formation of link-stabilized aggregates. The percentage aggregate was found to be related linearly to the amount of purified bovine link protein added in the 20-120 ng range. The assay was used to monitor loss of link-protein activity during heat denaturation and to measure binding of link protein by purified proteoglycan monomer.

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Year:  1985        PMID： 4091812      PMCID： PMC1152924          DOI： 10.1042/bj2320613

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  23 in total

1.  Proteoglycans from bovine nasal cartilage. Properties of a soluble form of link protein.

Authors:  L H Tang; L Rosenberg; A Reiner; A R Poole
Journal:  J Biol Chem       Date:  1979-10-25       Impact factor: 5.157

2.  The assay of xylosyltransferase in cartilage extracts. A modified procedure for preparation of Smith-degraded proteoglycan.

Authors:  J D Sandy
Journal:  Biochem J       Date:  1979-02-01       Impact factor: 3.857

3.  The specific interaction of hyaluronic acid with cartillage proteoglycans.

Authors:  T E Hardingham; H Muir
Journal:  Biochim Biophys Acta       Date:  1972-09-15

4.  The interaction of link proteins with proteoglycan monomers in the absence of hyaluronic acid.

Authors:  B Caterson; J Baker
Journal:  Biochem Biophys Res Commun       Date:  1978-02-14       Impact factor: 3.575

5.  The isolation and characterization of the link proteins from proteoglycan aggregates of bovine nasal cartilage.

Authors:  J R Baker; B Caterson
Journal:  J Biol Chem       Date:  1979-04-10       Impact factor: 5.157

6.  Radioimmunoassay of the link proteins associated with bovine nasal cartilage proteoglycan.

Authors:  B Caterson; J R Baker; D Levitt; J W Paslay
Journal:  J Biol Chem       Date:  1979-10-10       Impact factor: 5.157

7.  The role of link-protein in the structure of cartilage proteoglycan aggregates.

Authors:  T E Hardingham
Journal:  Biochem J       Date:  1979-01-01       Impact factor: 3.857

8.  Structure and interactions of cartilage proteoglycan binding region and link protein.

Authors:  F Bonnet; D G Dunham; T E Hardingham
Journal:  Biochem J       Date:  1985-05-15       Impact factor: 3.857

9.  Binding of oligosaccharides of hyaluronic acid to proteoglycans.

Authors:  T E Hardingham; H Muir
Journal:  Biochem J       Date:  1973-12       Impact factor: 3.857

10.  Proteoglycans from bovine nasal cartilage. Immunochemical studies of link protein.

Authors:  A R Poole; A Reiner; L H Tang; L Rosenberg
Journal:  J Biol Chem       Date:  1980-10-10       Impact factor: 5.157
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