Literature DB >> 4091661

Modification and standardization of the culture of early postimplantation embryos for toxicological studies.

S Klug, C Lewandowski, D Neubert.   

Abstract

The method of culturing "whole" rat embryos (days 9.5-11.5 of gestation, i.e. at the early stage of organogenesis) as modified and standardized in our laboratory is presented; We have succeeded in using bovine serum as culture medium instead of rat serum as recommended in the original procedure. Experimental conditions are described for obtaining reproducible results; An improved scoring system was developed which, in connection with a computerized documentation, greatly facilitates the evaluation of the data.

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Year:  1985        PMID： 4091661     DOI： 10.1007/BF00348314

Source DB:  PubMed          Journal:  Arch Toxicol        ISSN： 0340-5761            Impact factor:   5.153


  3 in total

Review 1.  Whole-embryo culture and the study of mammalian embryos during organogenesis.

Authors:  D A New
Journal:  Biol Rev Camb Philos Soc       Date:  1978-02

2.  Successful culture of rat embryos on human serum: use in the detection of teratogens.

Authors:  C L Chatot; N W Klein; J Piatek; L J Pierro
Journal:  Science       Date:  1980-03-28       Impact factor: 47.728

3.  Quantitation of rat embryonic development in vitro: a morphological scoring system.

Authors:  N A Brown; S Fabro
Journal:  Teratology       Date:  1981-08
  3 in total
  21 in total

1.  A simple method of culture of 11.5-day-old rat embryos in DMEM/F12 and 20% fetal bovine serum.

Authors:  A Ornoy; S Yacobi; P Yaffee
Journal:  J Anat       Date:  2003-10       Impact factor: 2.610

Review 2.  Alternative models in developmental toxicology.

Authors:  Hyung-yul Lee; Amy L Inselman; Jyotshnabala Kanungo; Deborah K Hansen
Journal:  Syst Biol Reprod Med       Date:  2012-02       Impact factor: 3.061

3.  Effects of valproic acid, some of its metabolites and analogues on prenatal development of rats in vitro and comparison with effects in vivo.

Authors:  S Klug; C Lewandowski; F Zappel; H J Merker; H Nau; D Neubert
Journal:  Arch Toxicol       Date:  1990       Impact factor: 5.153

4.  Radioactive labeling of proteins in cultured postimplantation mouse embryos. I. Influence of the embryo preparation method.

Authors:  J Nowak; J Klose
Journal:  In Vitro Cell Dev Biol       Date:  1989-07

5.  Pharmacokinetic aspects of drug effects in vitro: effects of serum protein binding on concentration and teratogenicity of valproic acid and 2-en-valproic acid in whole embryos in culture.

Authors:  C Lewandowski; S Klug; H Nau; D Neubert
Journal:  Arch Toxicol       Date:  1986-04       Impact factor: 5.153

6.  Teratogenicity of acyclovir in rats.

Authors:  R Stahlmann; S Klug; C Lewandowski; I Chahoud; G Bochert; H J Merker; D Neubert
Journal:  Infection       Date:  1987 Jul-Aug       Impact factor: 3.553

7.  Bcl-2 expression in a diabetic embryopathy model in presence of polyamines.

Authors:  Gladys Chirino-Galindo; D Eber Hernández-Hernández; L Coral Reyes-Mateos; Ricardo Mejía-Zepeda; Martha Martínez-García; Martín Palomar-Morales
Journal:  In Vitro Cell Dev Biol Anim       Date:  2019-08-19       Impact factor: 2.416

8.  Effect of aciclovir on the development of the chick embryo in ovo.

Authors:  B Heinrich-Hirsch; D Neubert
Journal:  Arch Toxicol       Date:  1991       Impact factor: 5.153

9.  Effect of lithium on rat embryos in culture: growth, development, compartmental distribution and lack of a protective effect of inositol.

Authors:  S Klug; M Collins; T Nagao; H J Merker; D Neubert
Journal:  Arch Toxicol       Date:  1992       Impact factor: 5.153

10.  In vitro and in vivo studies on the prenatal toxicity of five virustatic nucleoside analogues in comparison to aciclovir.

Authors:  S Klug; C Lewandowski; H J Merker; R Stahlmann; L Wildi; D Neubert
Journal:  Arch Toxicol       Date:  1991       Impact factor: 5.153
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