Literature DB >> 4085449

Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.

D H Fisher, J Adams, R W Giese.   

Abstract

Methods are being developed for derivatizing trace amounts of DNA adducts for ultimate determination by gas chromatographic techniques. High-pressure liquid chromatography is used to optimize appropriate derivatization reactions for the determination of cytosine. A single vial reaction scheme involves acylation with electrophoric pentafluorobenzoylchloride followed by alkylation with dimethyl sulfate. Currently, the overall yield for this reaction starting with 50 nmole of cytosine is 59 +/- 4.6%.

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Year:  1985        PMID： 4085449      PMCID： PMC1568697          DOI： 10.1289/ehp.856267

Source DB:  PubMed          Journal:  Environ Health Perspect        ISSN： 0091-6765            Impact factor:   9.031


  2 in total

1.  Electrophore-labeling and alkylation of standards of nucleic acid pyrimidine bases for analysis by gas chromatography with electron-capture detection.

Authors:  A Nazareth; M Joppich; S Abdel-Baky; K O'Connell; A Sentissi; R W Giese
Journal:  J Chromatogr       Date:  1984-11-30

2.  Gas chromatography-mass spectrometry characteristics of methylated perfluoroacyl derivatives of cytosine and 5-methylcytosine.

Authors:  G B Mohamed; A Nazareth; M J Hayes; R W Giese; P Vouros
Journal:  J Chromatogr       Date:  1984-11-30
  2 in total

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